Silk fibroin peptide self-assembled nanofibers delivered naringenin to alleviate cisplatin-induced acute kidney injury by inhibiting mtDNA-cGAS-STING pathway

Silk fibroin (SF) has excellent biocompatibility and biodegradability as a biomaterial. The purity and molecular weight distribution of silk fibroin peptide (SFP) make it more suitable for medical application. In this study, SFP nanofibers (molecular weight ∼30kD) were prepared through CaCl2/H2O/C2H...

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Published in:Food and chemical toxicology 2023-07, Vol.177, p.113844-113844, Article 113844
Main Authors: Liu, Shuai, Gao, Xintao, Yin, Yulan, Wang, Jing, Dong, Kehong, Shi, Dayong, Wu, Xiaochen, Guo, Chuanlong
Format: Article
Language:English
Subjects:
Acute kidney injury
Acute Kidney Injury - chemically induced
Acute Kidney Injury - prevention & control
Animals
cGAS-STING pathway
Cisplatin - toxicity
DNA, Mitochondrial - metabolism
Fibroins
Mice
Mitophagy
Nanofibers
Naringin
Nucleotidyltransferases - metabolism
Peptides - chemistry
Peptides - pharmacology
Silk fibroin peptide
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Summary:Silk fibroin (SF) has excellent biocompatibility and biodegradability as a biomaterial. The purity and molecular weight distribution of silk fibroin peptide (SFP) make it more suitable for medical application. In this study, SFP nanofibers (molecular weight ∼30kD) were prepared through CaCl2/H2O/C2H5OH solution decomposition and dialysis, and adsorbed naringenin (NGN) to obtain SFP/NGN NFs. In vitro results showed that SFP/NGN NFs increased the antioxidant activity of NGN and protected HK-2 cells from cisplatin-induced damage. In vivo results also showed that SFP/NGN NFs protected mice from cisplatin-induced acute kidney injury (AKI). The mechanism results showed that cisplatin induced mitochondrial damage, as well as increased mitophagy and mtDNA release, which activated the cGAS-STING pathway and induced the expression of inflammatory factors such as IL-6 and TNF-α. Interestingly, SFP/NGN NFs further activated mitophagy and inhibited mtDNA release and cGAS-STING pathway. Demonstrated that mitophagy-mtDNA-cGAS-STING signal axis was involved in the kidney protection mechanism of SFP/NGN NFs. In conclusion, our study confirmed that SFP/NGN NFs are candidates for protection of cisplatin-induced AKI, which is worthy of further study. [Display omitted]
ISSN:0278-6915
1873-6351
DOI:10.1016/j.fct.2023.113844