Optical Tomography of Polymeric Microsphere Layers Using Confocal Raman Microscopy

In confocal Raman microscopy, depth profiling is a key application that enables analysis of the structural and chemical composition and size of three-dimensional (3D) transparent objects. However, the precise interpretation of a probed sample's Raman depth profile measurement can be significant...

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Bibliographic Details
Published in:Applied spectroscopy 2023-07, Vol.77 (7), p.744-752
Main Authors: Syring, Alina, Wang, Zunhao, Wundrack, Stefan, Stosch, Rainer, Voss, Tobias
Format: Article
Language:English
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Summary:In confocal Raman microscopy, depth profiling is a key application that enables analysis of the structural and chemical composition and size of three-dimensional (3D) transparent objects. However, the precise interpretation of a probed sample's Raman depth profile measurement can be significantly affected by both its size and surrounding objects. This study provides a more comprehensive understanding of the observed optical effects at the interface between polymer spheres and different substrates. Ray- and wave-optical simulations support our results. We derive a correction factor that, depending on the instrumental configuration, allows us to determine the nominal dimensions of the scanned objects more accurately from Raman depth profiles. Our studies support the need for careful consideration when employing depth profiling in confocal Raman microscopy for nondestructive, quantitative tomography of 3D objects. Graphical Abstract This is a visual representation of the abstract.
ISSN:0003-7028
1943-3530
DOI:10.1177/00037028231175922