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Regulation of Cat8 in energy metabolic balance and glucose tolerance in Saccharomyces cerevisiae
Cat8 is a C6 zinc cluster transcription activator in yeast. It is generally recognized that the transcription of CAT8 is inhibited and that Cat8 is inactive in the presence of high concentrations of glucose. However, our recent study found that constitutively overexpressed Cat8 played a regulatory r...
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Published in: | Applied microbiology and biotechnology 2023-07, Vol.107 (14), p.4605-4619 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Cat8 is a C6 zinc cluster transcription activator in yeast. It is generally recognized that the transcription of
CAT8
is inhibited and that Cat8 is inactive in the presence of high concentrations of glucose. However, our recent study found that constitutively overexpressed Cat8 played a regulatory role in
Saccharomyces cerevisiae
in the presence of 20 g/L glucose
.
To explore the regulatory network of Cat8 at high glucose concentrations,
CAT8
was both overexpressed and deleted in this study. Cell growth and glucose consumption in different media were significantly accelerated by the deletion of
CAT8
, while the lag period was greatly shortened. RNA-seq and genetic modification showed that the deletion of
CAT8
changed the type of energy metabolism in yeast cells. Many genes related to the mitochondrial respiratory chain were downregulated, resulting in a reduction in aerobic respiration and the tricarboxylic acid cycle. Meanwhile, both the energy supply of anaerobic ethanol fermentation and the Crabtree effect of
S. cerevisiae
were enhanced by the deletion of
CAT8
.
CAT8
knockout cells show a higher sugar uptake rate, a higher cell growth rate, and higher tolerance to glucose than the wild-type strain YS58. This study expands the understanding of the regulatory network of Cat8 and provides guidance for modulating yeast cell growth.
Key points
•
The deletion of CAT8 promoted cell growth of S. cerevisiae.
•
Transcriptome analysis revealed the regulation network of Cat8 under 1% glucose condition.
•
CAT8 deletion increases the glucose tolerance of cells by enhancing the Crabtree effect. |
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ISSN: | 0175-7598 1432-0614 |
DOI: | 10.1007/s00253-023-12593-2 |