Separation and identification of different therapeutic pharmaceutical drugs on humic acid‐based HPLC column: Method optimization using central composite design

Multimode chromatographic separations are highly desirable in pharmaceutical and environmental sciences. Current study deals with the application of newly developed mixed‐mode end capped‐immobilized humic acid onto an aminopropyl silica based chromatographic column for separation and identification...

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Bibliographic Details
Published in:Journal of separation science 2023-07, Vol.46 (14), p.e2200914-n/a
Main Authors: Ayyildiz, Hamide Filiz, Memon, Fakhar N., Mangi, Irfan Ali, Kara, Huseyin
Format: Article
Language:English
Subjects:
Acetonitrile
Aminopropyl silica
central composite design
Chromatography
Design optimization
Drugs
Flow velocity
High performance liquid chromatography
humic acid
Humic acids
Industrial applications
Ions
Linearity
Pharmaceuticals
Sensitivity
Separation
therapeutic drugs
Variance analysis
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Summary:Multimode chromatographic separations are highly desirable in pharmaceutical and environmental sciences. Current study deals with the application of newly developed mixed‐mode end capped‐immobilized humic acid onto an aminopropyl silica based chromatographic column for separation and identification of six drugs belonging to different therapeutic groups for its applicability in pharmaceutical industries. For this, central composite design was used to evaluate the separation and resolution by optimization of three most effective parameters (acetonitrile%, flow rate, and pH of mobile phase). Second‐order quadratic model was used to evaluate their effect on resolution of peaks; the probability value ( 1%), linear (R2 = 0.99), and highly selective method. Moreover, the limit of detection/quantification values revealed acceptable sensitivity of the method. The developed column was compared for its efficiency with a commercially available column and found to be highly applicable for industrial applications.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.202200914