Microbial community composition analysis to decipher the possible role of inherent bacteria for in-situ arsenic (As) bioremediation

• Published in: 3 Biotech 2023-06, Vol.13 (6), p.214-214, Article 214
• Main Authors: Biswas, Rimi, Paul, Dhiraj, Maity, Sourav, Sarkar, Angana
• Format: Article
• Language: English
• Subjects: Agriculture; Aquifers; Arsenic; Arsenite; Biogeochemical cycles; Biogeochemistry; Bioinformatics; Biomaterials; Bioremediation; Biotechnology; Cancer Research; Chemistry; Chemistry and Materials Science; Community composition; Community structure; Cyanobacteria; Detoxification; Groundwater; Microbiomes; Microorganisms; Original Article; Oxidation; Proteobacteria; Species richness; Stem Cells; Substrates; Uranium
• ISSN: 2190-572X; 2190-5738
• DOI: 10.1007/s13205-023-03612-0

Biogeochemical reduction and mobilization of sediment-bound arsenic (As) is the major concern for widespread groundwater As contamination in the middle Gangetic plains. The present work examines a microcosm based bio-stimulation study and substrate amendments over 45 days to analyze the bacterial community structure and distribution to indicate the possible in-situ bioremediation strategy in the area. Initially, Bacterial phyla Proteobacteria was predominantly present in all the samples, followed by Actinobacteria , Bacteroidetes, and Firmicutes whereas Cyanobacteria was noted as the minor group. In genus level, Delftia , Acinetobacter, Lysobacter, Bacillus, and Pseudomonas were the major groups of bacteria in the As-rich aquifer system, while Planctomycetes dominated the bio-stimulated samples, followed by a minute portion of Proteobacteria . Alpha diversity and Chaol curve further determined the species richness in the samples with an As tolerant capacity of 152.28 ppb. The presence of γ- Proteobacteria as the dominating member in high As-content water indicated their predominant role in As mobilization, whereas, dominance of α-Proteobacterial members in low As-content water indicated their involvement in As detoxification. The complete change in microbial community structure within the bio-stimulated conditions indicated the extensive role of arsenite-oxidizing microbial communities within different levels of As-contaminated areas in Bihar that will enlighten the significant role of these communities in As-biogeochemical cycle.