Genomic insight into Algoriphagus limi sp. nov. isolated from a mudflat on the Yellow Sea coast

Abstract A Gram-negative, non-motile, and reddish-orange colored bacterium, designated CAU 1643T, was isolated from a mudflat collected in Ganghwa Island, Republic of Korea. The bacterium was found to grow optimally at 30°C, pH 9.0–9.5, and with 0%–1% (w/v) NaCl. The highest 16S rRNA gene sequence s...

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Bibliographic Details
Published in:FEMS microbiology letters 2023-01, Vol.370
Main Authors: Ahn, Soyeon, Kim, Jong-Hwa, Yoon, Jung-Hoon, Lee, Jung-Sook, Sukhoom, Ampaitip, Kim, Wonyong
Format: Article
Language:English
Subjects:
Bacteria
Bacteroidetes
Biotechnology
Carbohydrates
Deoxyribonucleic acid
DNA
DNA, Bacterial - genetics
Gene clusters
Genes
Genomes
Genomics
Hybridization
Medicine
Menaquinones
Metabolites
Mud flats
National libraries
New species
Nucleotides
Phylogenetics
Phylogeny
Quinones
RNA, Ribosomal, 16S - genetics
rRNA 16S
Seawater - microbiology
Secondary metabolites
Sequence Analysis, DNA
Sodium chloride
Taxonomy
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Summary:Abstract A Gram-negative, non-motile, and reddish-orange colored bacterium, designated CAU 1643T, was isolated from a mudflat collected in Ganghwa Island, Republic of Korea. The bacterium was found to grow optimally at 30°C, pH 9.0–9.5, and with 0%–1% (w/v) NaCl. The highest 16S rRNA gene sequence similarities to the bacterium were Algoriphagus kandeliae XY-J91T (97.9%), A. aquimaris F21T (97.1%), A. formosus XAY3209T (97.0%), and A. marincola DSM 16067T (96.2%). The DNA G + C content of the type strain was 40.35 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain CAU 1643T and the reference strains were below the threshold value for species demarcation. The predominant cellular fatty acids were iso-C15:0, iso-C17:0 3-OH, and Summed Feature 9. The major respiratory quinone was menaquinone-7. The genome showed three putative biosynthetic gene clusters that are responsible for different secondary metabolites. Moreover, CAU 1643T contains 72 genes that encode carbohydrate-active enzymes. Based on phenotypic, phylogenetic, and chemotaxonomic evidence, strain CAU 1643T represents novel species in the genus Algoriphagus, for which the name A. limi sp. nov. is proposed. The type strain is CAU 1643T ( = KCTC 92080T, = MCCC 1K07150T). We have isolated a new Algoriphagus species, described here as A. limi sp. nov.
ISSN:1574-6968
0378-1097
1574-6968
DOI:10.1093/femsle/fnad045