Aronia melanocarpa (Michx.) Elliott 1821 Extract Has Moderate Ameliorative Influence on Biochemical and Hematological Parameters in Gentamicin-Induced Nephropathy in Wistar Rats

• Published in: Applied biochemistry and biotechnology 2024-02, Vol.196 (2), p.896-908
• Main Authors: Fočak, Muhamed, Mitrašinović-Brulić, Maja, Suljević, Damir
• Format: Article
• Language: English
• Subjects: Aminoglycoside antibiotics; Aminoglycosides; Anemia; Anti-inflammatory agents; Aronia melanocarpa; Bacterial diseases; Biochemistry; Biotechnology; Chemistry; Chemistry and Materials Science; Creatinine; Gentamicin; Globulins; Hematology; Hemoglobin; Inflammation; Kidneys; Leukocytes (neutrophilic); Nephropathy; Neutrophils; Original Article; Parameters; Renal failure; Urea; Ureas
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-023-04573-z

Gentamicin (GM) is an aminoglycoside antibiotic used to treat bacterial infections. Nephrotoxicity refers to the impairments of the kidneys caused by the use of GM and can result in decreased kidney function and in severe cases, kidney failure. Aronia melanocarpa extract (AME), also known as the black chokeberry, has been used for its protective effects on the kidneys. AME concentration of 3.38 mg/kg (max antioxidant activity in vitro) was used to determine its effectiveness against induced nephropathy during 30 days. GM treatment caused significant hypoalbuminemia and high values of globulins, creatinine, and urea compared to the control group. GM application lead to hemolysis occurrence, echinocytosis, and platelets aggregation. Significantly high values of segmented neutrophils and low values of non-segmented neutrophils were recorded in the blood of rats treated with chokeberry extract (AME). In the pre-treatment (AME + GM), severe hypochromic anemia and a significant improvement in hematological parameters, as well as a reduction of anemia in the post-treatment (GM + AME), were noted. Post-treatment AME also significantly regulates urea and creatinine values. Statistically significantly low hemoglobin values were found in all groups treated with AME. Current study suggests that compounds in the AME have a moderate beneficial effect against renal injury and anti-inflammatory properties that may help protect the kidneys from injury caused by GM.