Interactions between polysialic acid and dopamine-lead compounds as revealed by biochemical and in silico docking simulation analyses

Polysialic acid is an important glyco-epitope in vertebrate brains, while altered expressions of polySia and biosynthetic enzyme have been reported in brain diseases such as schizophrenia and depression. Recently, the binding between polySia and dopamine and the involvement of this in Akt signaling...

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Bibliographic Details
Published in:Glycoconjugate journal 2023-08, Vol.40 (4), p.461-471
Main Authors: Hayakawa, Kaito, Hane, Masaya, Hamagami, Hiroki, Imai, Miki, Tanaka, Hiroshi, Kitajima, Ken, Sato, Chihiro
Format: Article
Language:English
Subjects:
Acids
Affinity
Affinity chromatography
AKT protein
Antibodies
Biochemistry
Biomedical and Life Sciences
Bipolar disorder
Brain - metabolism
Brain research
Chromatography
Dopamine
Enzyme-linked immunosorbent assay
Enzymes
Epitopes
Life Sciences
Ligands
Mental disorders
Molecular modelling
Mutation
Pathology
Polysialic acid
Schizophrenia
Sialic Acids - metabolism
Signal Transduction
Simulation
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Summary:Polysialic acid is an important glyco-epitope in vertebrate brains, while altered expressions of polySia and biosynthetic enzyme have been reported in brain diseases such as schizophrenia and depression. Recently, the binding between polySia and dopamine and the involvement of this in Akt signaling has been demonstrated. However, the molecular mechanism underlying the binding of polySia and dopamine remains unknown. Therefore, here, we demonstrated the interaction between dopamine and polySia using frontal affinity chromatography alongside docking simulations. In addition, we prepared dopamine-lead compounds to understand the detailed molecular basis of polySia binding by frontal affinity chromatography, enzyme-linked immunosorbent assay, and docking simulations.
ISSN:0282-0080
1573-4986
1573-4986
DOI:10.1007/s10719-023-10119-6