Ca2+-responsive phospholipid-binding BONZAI genes confer a novel role for cotton resistance to Verticillium wilt

Verticillium wilt which produced by the soil-borne fungus Verticillium dahliae is an important biotic threat that limits cotton ( Gossypium hirsutum ) growth and agricultural productivity. It is very essential to explore new genes for the generation of V. dahliae resistance or tolerance cotton varie...

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Published in:Plant molecular biology 2023-07, Vol.112 (4-5), p.247-259
Main Authors: Hao, Xiaoyan, Gao, Shengqi, Luo, Tiantian, Zhao, Zhun, Shao, Wukui, Li, Jianping, Hu, Wenran, Huang, Quansheng
Format: Article
Language:English
Subjects:
Agricultural production
Biochemistry
Biomedical and Life Sciences
Calcium signalling
Cell death
Cellular stress response
Cotton
Disease resistance
Gene regulation
Gene silencing
Genomes
Gossypium hirsutum
Hydrogen peroxide
Kinases
Life Sciences
Pathogens
Phospholipids
Plant diseases
Plant immunity
Plant Pathology
Plant Sciences
Soil microorganisms
Verticillium
Verticillium wilt
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Summary:Verticillium wilt which produced by the soil-borne fungus Verticillium dahliae is an important biotic threat that limits cotton ( Gossypium hirsutum ) growth and agricultural productivity. It is very essential to explore new genes for the generation of V. dahliae resistance or tolerance cotton varieties. Ca 2+ signaling as a secondary messenger is involved in pathogen stress response. Despite Ca 2+ -responsive phospholipid-binding BONZAI ( BON ) genes have intensively been investigated in Arabidopsis , their function has not still been characterized in cotton. Here, we showed that three copies of GhBON1 , two copies of GhBON2 and GhBON3 were found from the genome sequences of upland cotton. The expression of GhBON1 was inducible to V. dahliae . Knocking down of GhBON1 , GhBON2 and GhBON3 using virus induced gene silencing (VIGS) each increased up-regulation of defense responses in cotton. These GhBON1 , GhBON2 and GhBON3 -silenced plants enhanced resistance to V. dahliae accompanied by higher burst of hydrogen peroxide and decreased cell death and had more effect on the up-regulation of defense response genes. Further analysis revealed that GhBON1 could interacts with BAK1-interacting receptor-like kinase 1 ( GhBIR1 ) and pathogen-associated molecular pattern (PAMP) receptor regulator BAK1 ( GhBAK1 ) at plasma membrane. Our study further reveals that plant Ca 2+ -responsive phospholipid-binding BONZAI genes negatively regulate Verticillium wilt with the conserved function in response to disease resistance or plant immunity. Key message Plant Ca 2+ -responsive phospholipid-binding BONZAI genes negatively regulate cotton disease resistance to V. dahliae .
ISSN:0167-4412
1573-5028
DOI:10.1007/s11103-023-01359-z