Kinetic model for lactose hydrolysis in a recirculation hollow-fibre bioreactor

Enzymatic hydrolysis of lactose by *b-galactosidase (Lactozym) has been studied in a jacketed stirred-tank reactor (JSTR) and in a recirculation hollow-fibre bioreactor (RHFB). The hollow-fibre module was manufactured with modified cellulose-acetate membrane (Cuprophan). The variables tested were: t...

Full description

Saved in:
Bibliographic Details
Published in:Chemical engineering science 2004-01, Vol.59 (2), p.397-405.
Main Authors: Jurado, E, Camacho, F, Luzon, G, Vicaria, J M
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Enzymatic hydrolysis of lactose by *b-galactosidase (Lactozym) has been studied in a jacketed stirred-tank reactor (JSTR) and in a recirculation hollow-fibre bioreactor (RHFB). The hollow-fibre module was manufactured with modified cellulose-acetate membrane (Cuprophan). The variables tested were: temperature (25-40DGC), lactose concentration (0.0278-0.208 M) and enzyme concentration (0.100-41.51 g l(super -1)). Within the experimental range analysed, the conversions in the RHFB, were lower than in the JSTR. A kinetic model is proposed for enzymatic hydrolysis of lactose in the RHFB, which considers competitive inhibition by the product (galactose) and reversible adsorption of the enzyme-galactose complex on the hollow-fibre membrane. The adsorption enthalpy of the enzyme-galactose complex onto the membrane was determined as being 77 5 kJ mol(super -1).
ISSN:0009-2509
DOI:10.1016/j.ces.2003.09.035