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Combined detection of SDC2/ADHFE1/PPP2R5C methylation in stool DNA for colorectal cancer screening
Background Colorectal cancer (CRC) is a disease of global concern, and its increasing incidence suggests the need for early and accurate diagnosis. The aim of this study was to investigate the value of combined detection of SDC2, ADHFE1 and PPP2R5C gene methylation in stool samples for early CRC scr...
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| Published in: | Journal of cancer research and clinical oncology 2023-09, Vol.149 (12), p.10241-10253 |
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| container_end_page | 10253 |
| container_issue | 12 |
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| container_title | Journal of cancer research and clinical oncology |
| container_volume | 149 |
| creator | Li, Ben Liu, Shanglong Gao, Yuan Zheng, Longbo Lu, Yun |
| description | Background
Colorectal cancer (CRC) is a disease of global concern, and its increasing incidence suggests the need for early and accurate diagnosis. The aim of this study was to investigate the value of combined detection of SDC2, ADHFE1 and PPP2R5C gene methylation in stool samples for early CRC screening.
Methods
Stool samples from patients with CRC (
n
= 105), advanced adenoma (AA) (
n
= 54), non-advanced adenoma (NA) (
n
= 57), hyperplastic or other polyps (HOP) (
n
= 47) or no evidence of disease (NED) (
n
= 100) were collected from September 2021 to September 2022. The methylation levels of SDC2, ADHFE1 and PPP2R5C were quantified by quantitative methylation-specific polymerase chain reaction (qMSP), and faecal immunochemical testing (FIT) was performed. The diagnostic value was assessed using reporter operating characteristic (ROC) curve analysis.
Results
The sensitivity of combined detection of SDC2/ADHFE1/PPP2R5C methylation in predicting CRC (0–IV) was 84.8%, the specificity was 98.0%, and the AUC was 0.930 (95% CI 0.889–0.970). Compared to FIT and serum tumour biomarkers, it showed better diagnostic performance for different stages of CRC.
Conclusion
The results of this study verified that the methylation levels of SDC2, ADHFE1 and PPP2R5C in stool DNA were significantly increased in CRC patients. Combined detection of SDC2/ADHFE1/PPP2R5C methylation is a potential non-invasive diagnostic method for CRC and precancerous lesion screening.
Clinical trial registration
Chinese Clinical Trials Registry, ChiCTR2100046662, registered on 26 May 2021, prospective registration. |
| doi_str_mv | 10.1007/s00432-023-04943-4 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2822367935</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2849394559</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-af74808bb827279ee18a73bf2a5fb96acf90af361077046cc8c80aab4a3d31fc3</originalsourceid><addsrcrecordid>eNp9kTtPwzAUhS0EoqXwBxiQJRaWUD_jZKxSCkgIKh6z5bh2SZXYxU4H_j0u5SExMFnX_s7x0T0AnGJ0iRES44gQoyRDhGaIlYxmbA8M8fYKU8r3wRBhgTNOcD4ARzGuUJq5IIdgQAURiOVoCOrKd3XjzAIuTG9033gHvYVP04qMJ9Ob2RUez-dz8sgr2Jn-9b1Vn0jjYOy9b-H0fgKtD1D71oekVy3UymkTYNTBGNe45TE4sKqN5uTrHIGX2dVzdZPdPVzfVpO7TFPB-0xZwQpU1HVBUrrSGFwoQWtLFLd1mSttS6QszTES2-xaF7pAStVM0QXFVtMRuNj5roN_25jYy66J2rStcsZvoiQFITQXJeUJPf-DrvwmuJQuUaykJeO8TBTZUTr4GIOxch2aToV3iZHcNiB3DcjUgPxsQLIkOvuy3tSdWfxIvleeALoDYnpySxN-__7H9gNY_I7M</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2849394559</pqid></control><display><type>article</type><title>Combined detection of SDC2/ADHFE1/PPP2R5C methylation in stool DNA for colorectal cancer screening</title><source>Springer Link</source><creator>Li, Ben ; Liu, Shanglong ; Gao, Yuan ; Zheng, Longbo ; Lu, Yun</creator><creatorcontrib>Li, Ben ; Liu, Shanglong ; Gao, Yuan ; Zheng, Longbo ; Lu, Yun</creatorcontrib><description>Background
Colorectal cancer (CRC) is a disease of global concern, and its increasing incidence suggests the need for early and accurate diagnosis. The aim of this study was to investigate the value of combined detection of SDC2, ADHFE1 and PPP2R5C gene methylation in stool samples for early CRC screening.
Methods
Stool samples from patients with CRC (
n
= 105), advanced adenoma (AA) (
n
= 54), non-advanced adenoma (NA) (
n
= 57), hyperplastic or other polyps (HOP) (
n
= 47) or no evidence of disease (NED) (
n
= 100) were collected from September 2021 to September 2022. The methylation levels of SDC2, ADHFE1 and PPP2R5C were quantified by quantitative methylation-specific polymerase chain reaction (qMSP), and faecal immunochemical testing (FIT) was performed. The diagnostic value was assessed using reporter operating characteristic (ROC) curve analysis.
Results
The sensitivity of combined detection of SDC2/ADHFE1/PPP2R5C methylation in predicting CRC (0–IV) was 84.8%, the specificity was 98.0%, and the AUC was 0.930 (95% CI 0.889–0.970). Compared to FIT and serum tumour biomarkers, it showed better diagnostic performance for different stages of CRC.
Conclusion
The results of this study verified that the methylation levels of SDC2, ADHFE1 and PPP2R5C in stool DNA were significantly increased in CRC patients. Combined detection of SDC2/ADHFE1/PPP2R5C methylation is a potential non-invasive diagnostic method for CRC and precancerous lesion screening.
Clinical trial registration
Chinese Clinical Trials Registry, ChiCTR2100046662, registered on 26 May 2021, prospective registration.</description><identifier>ISSN: 0171-5216</identifier><identifier>EISSN: 1432-1335</identifier><identifier>DOI: 10.1007/s00432-023-04943-4</identifier><identifier>PMID: 37270460</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adenoma ; Cancer Research ; Cancer screening ; Clinical trials ; Colorectal cancer ; Colorectal carcinoma ; CTR ; CTR2100046662 ; DNA methylation ; Feces ; Hematology ; Internal Medicine ; Medical screening ; Medicine ; Medicine & Public Health ; Oncology ; Polyps ; Tumors</subject><ispartof>Journal of cancer research and clinical oncology, 2023-09, Vol.149 (12), p.10241-10253</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-af74808bb827279ee18a73bf2a5fb96acf90af361077046cc8c80aab4a3d31fc3</citedby><cites>FETCH-LOGICAL-c375t-af74808bb827279ee18a73bf2a5fb96acf90af361077046cc8c80aab4a3d31fc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37270460$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Ben</creatorcontrib><creatorcontrib>Liu, Shanglong</creatorcontrib><creatorcontrib>Gao, Yuan</creatorcontrib><creatorcontrib>Zheng, Longbo</creatorcontrib><creatorcontrib>Lu, Yun</creatorcontrib><title>Combined detection of SDC2/ADHFE1/PPP2R5C methylation in stool DNA for colorectal cancer screening</title><title>Journal of cancer research and clinical oncology</title><addtitle>J Cancer Res Clin Oncol</addtitle><addtitle>J Cancer Res Clin Oncol</addtitle><description>Background
Colorectal cancer (CRC) is a disease of global concern, and its increasing incidence suggests the need for early and accurate diagnosis. The aim of this study was to investigate the value of combined detection of SDC2, ADHFE1 and PPP2R5C gene methylation in stool samples for early CRC screening.
Methods
Stool samples from patients with CRC (
n
= 105), advanced adenoma (AA) (
n
= 54), non-advanced adenoma (NA) (
n
= 57), hyperplastic or other polyps (HOP) (
n
= 47) or no evidence of disease (NED) (
n
= 100) were collected from September 2021 to September 2022. The methylation levels of SDC2, ADHFE1 and PPP2R5C were quantified by quantitative methylation-specific polymerase chain reaction (qMSP), and faecal immunochemical testing (FIT) was performed. The diagnostic value was assessed using reporter operating characteristic (ROC) curve analysis.
Results
The sensitivity of combined detection of SDC2/ADHFE1/PPP2R5C methylation in predicting CRC (0–IV) was 84.8%, the specificity was 98.0%, and the AUC was 0.930 (95% CI 0.889–0.970). Compared to FIT and serum tumour biomarkers, it showed better diagnostic performance for different stages of CRC.
Conclusion
The results of this study verified that the methylation levels of SDC2, ADHFE1 and PPP2R5C in stool DNA were significantly increased in CRC patients. Combined detection of SDC2/ADHFE1/PPP2R5C methylation is a potential non-invasive diagnostic method for CRC and precancerous lesion screening.
Clinical trial registration
Chinese Clinical Trials Registry, ChiCTR2100046662, registered on 26 May 2021, prospective registration.</description><subject>Adenoma</subject><subject>Cancer Research</subject><subject>Cancer screening</subject><subject>Clinical trials</subject><subject>Colorectal cancer</subject><subject>Colorectal carcinoma</subject><subject>CTR</subject><subject>CTR2100046662</subject><subject>DNA methylation</subject><subject>Feces</subject><subject>Hematology</subject><subject>Internal Medicine</subject><subject>Medical screening</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Oncology</subject><subject>Polyps</subject><subject>Tumors</subject><issn>0171-5216</issn><issn>1432-1335</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kTtPwzAUhS0EoqXwBxiQJRaWUD_jZKxSCkgIKh6z5bh2SZXYxU4H_j0u5SExMFnX_s7x0T0AnGJ0iRES44gQoyRDhGaIlYxmbA8M8fYKU8r3wRBhgTNOcD4ARzGuUJq5IIdgQAURiOVoCOrKd3XjzAIuTG9033gHvYVP04qMJ9Ob2RUez-dz8sgr2Jn-9b1Vn0jjYOy9b-H0fgKtD1D71oekVy3UymkTYNTBGNe45TE4sKqN5uTrHIGX2dVzdZPdPVzfVpO7TFPB-0xZwQpU1HVBUrrSGFwoQWtLFLd1mSttS6QszTES2-xaF7pAStVM0QXFVtMRuNj5roN_25jYy66J2rStcsZvoiQFITQXJeUJPf-DrvwmuJQuUaykJeO8TBTZUTr4GIOxch2aToV3iZHcNiB3DcjUgPxsQLIkOvuy3tSdWfxIvleeALoDYnpySxN-__7H9gNY_I7M</recordid><startdate>20230901</startdate><enddate>20230901</enddate><creator>Li, Ben</creator><creator>Liu, Shanglong</creator><creator>Gao, Yuan</creator><creator>Zheng, Longbo</creator><creator>Lu, Yun</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20230901</creationdate><title>Combined detection of SDC2/ADHFE1/PPP2R5C methylation in stool DNA for colorectal cancer screening</title><author>Li, Ben ; Liu, Shanglong ; Gao, Yuan ; Zheng, Longbo ; Lu, Yun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-af74808bb827279ee18a73bf2a5fb96acf90af361077046cc8c80aab4a3d31fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Adenoma</topic><topic>Cancer Research</topic><topic>Cancer screening</topic><topic>Clinical trials</topic><topic>Colorectal cancer</topic><topic>Colorectal carcinoma</topic><topic>CTR</topic><topic>CTR2100046662</topic><topic>DNA methylation</topic><topic>Feces</topic><topic>Hematology</topic><topic>Internal Medicine</topic><topic>Medical screening</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Oncology</topic><topic>Polyps</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Ben</creatorcontrib><creatorcontrib>Liu, Shanglong</creatorcontrib><creatorcontrib>Gao, Yuan</creatorcontrib><creatorcontrib>Zheng, Longbo</creatorcontrib><creatorcontrib>Lu, Yun</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest research library</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cancer research and clinical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Ben</au><au>Liu, Shanglong</au><au>Gao, Yuan</au><au>Zheng, Longbo</au><au>Lu, Yun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combined detection of SDC2/ADHFE1/PPP2R5C methylation in stool DNA for colorectal cancer screening</atitle><jtitle>Journal of cancer research and clinical oncology</jtitle><stitle>J Cancer Res Clin Oncol</stitle><addtitle>J Cancer Res Clin Oncol</addtitle><date>2023-09-01</date><risdate>2023</risdate><volume>149</volume><issue>12</issue><spage>10241</spage><epage>10253</epage><pages>10241-10253</pages><issn>0171-5216</issn><eissn>1432-1335</eissn><abstract>Background
Colorectal cancer (CRC) is a disease of global concern, and its increasing incidence suggests the need for early and accurate diagnosis. The aim of this study was to investigate the value of combined detection of SDC2, ADHFE1 and PPP2R5C gene methylation in stool samples for early CRC screening.
Methods
Stool samples from patients with CRC (
n
= 105), advanced adenoma (AA) (
n
= 54), non-advanced adenoma (NA) (
n
= 57), hyperplastic or other polyps (HOP) (
n
= 47) or no evidence of disease (NED) (
n
= 100) were collected from September 2021 to September 2022. The methylation levels of SDC2, ADHFE1 and PPP2R5C were quantified by quantitative methylation-specific polymerase chain reaction (qMSP), and faecal immunochemical testing (FIT) was performed. The diagnostic value was assessed using reporter operating characteristic (ROC) curve analysis.
Results
The sensitivity of combined detection of SDC2/ADHFE1/PPP2R5C methylation in predicting CRC (0–IV) was 84.8%, the specificity was 98.0%, and the AUC was 0.930 (95% CI 0.889–0.970). Compared to FIT and serum tumour biomarkers, it showed better diagnostic performance for different stages of CRC.
Conclusion
The results of this study verified that the methylation levels of SDC2, ADHFE1 and PPP2R5C in stool DNA were significantly increased in CRC patients. Combined detection of SDC2/ADHFE1/PPP2R5C methylation is a potential non-invasive diagnostic method for CRC and precancerous lesion screening.
Clinical trial registration
Chinese Clinical Trials Registry, ChiCTR2100046662, registered on 26 May 2021, prospective registration.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>37270460</pmid><doi>10.1007/s00432-023-04943-4</doi><tpages>13</tpages></addata></record> |
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| ispartof | Journal of cancer research and clinical oncology, 2023-09, Vol.149 (12), p.10241-10253 |
| issn | 0171-5216 1432-1335 |
| language | eng |
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| subjects | Adenoma Cancer Research Cancer screening Clinical trials Colorectal cancer Colorectal carcinoma CTR CTR2100046662 DNA methylation Feces Hematology Internal Medicine Medical screening Medicine Medicine & Public Health Oncology Polyps Tumors |
| title | Combined detection of SDC2/ADHFE1/PPP2R5C methylation in stool DNA for colorectal cancer screening |
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