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Binary CuO\CoO nanoparticles inhibit biofilm formation and reduce the expression of papC and fimH genes in multidrug-resistant Klebsiella oxytoca
Background and aim Binary copper-cobalt oxide nanoparticles (CuO\CoO NPs) are modern kinds of antimicrobials, which may get a lot of interest in clinical application. This study aimed to detect the effect of the binary CuO\CoO NPs on the expression of papC and fimH genes in multidrug-resistant (MDR)...
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Published in: | Molecular biology reports 2023-07, Vol.50 (7), p.5969-5976 |
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Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Background and aim
Binary copper-cobalt oxide nanoparticles (CuO\CoO NPs) are modern kinds of antimicrobials, which may get a lot of interest in clinical application. This study aimed to detect the effect of the binary CuO\CoO NPs on the expression of
papC
and
fimH
genes in multidrug-resistant (MDR) isolates of
Klebsiella oxytoca
to reduce medication time and improve outcomes.
Methods
Ten isolates of
K. oxytoca
were collected and identified by different conventional tests besides PCR. Antibiotic sensitivity and biofilm-forming ability were carried out. The harboring of
papC
and
fimH
genes was also detected. The effect of binary CuO\CoO nanoparticles on the expression of
papC
and
fimH
genes was investigated.
Results
Bacterial resistance against cefotaxime and gentamicin was the highest (100%), while the lowest percentage of resistance was to amikacin (30%). Nine of the ten bacterial isolates had the ability to form a biofilm with different capacities. MIC for binary CuO\CoO NPs was 2.5 µg/mL. Gene expression of
papC
and
fimH
was 8.5- and 9-fold lower using the NPs.
Conclusion
Binary CuO\CoO NPs have a potential therapeutic effect against infections triggered by MDR
K. oxytoca
strains due to the NPs-related downregulation ability on the virulence genes of
K. oxytoca
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ISSN: | 0301-4851 1573-4978 |
DOI: | 10.1007/s11033-023-08447-9 |