Enhanced apoptotic index in hepatocytes, Kupffer cells, and inflammatory infiltrate showed positive correlation with hepatic lesion intensity, parasite load, and clinical status in naturally Leishmania-infected dogs

• Published in: Microbial pathogenesis 2023-08, Vol.181, p.106194-106194, Article 106194
• Main Authors: Verçosa, Bárbara Laurice Araujo, Muniz-Junqueira, Maria Imaculada, Mineiro, Ana Lys Bezerra Barradas, Costa, Francisco Assis Lima, Melo, Maria Norma, Vasconcelos, Anilton Cesar
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Canine leishmaniosis; Dog Diseases - parasitology; Dogs; Hepatocytes - pathology; Inflammation; Kupffer Cells - pathology; Leishmania infantum; Leishmaniasis; Leishmaniasis, Visceral - parasitology; Leishmaniasis, Visceral - veterinary; Liver; Parasite Load - veterinary
• ISSN: 0882-4010; 1096-1208
• DOI: 10.1016/j.micpath.2023.106194

It is unknown if Leishmania amastigote infections affect hepatocytes and Kupffer cell apoptosis, and the role played by apoptosis in liver lesions in leishmaniasis is still unclear. Clinically affected and subclinically infected dogs with leishmaniosis and uninfected controls were assessed. Parasite load, biochemical markers for evaluation of liver damage, morphometry (area, perimeter, number of inflammatory focus, major and minor diameters), apoptosis in hepatic tissue (hepatocytes, Kupffer cells, and inflammatory infiltrates) and cellularity in inflammatory foci were quantified. The parasite load in clinically affected dogs proved to be higher than in the other groups. All morphometric parameters (area, perimeter, number of inflammatory focus, major and minor diameters) from clinically affected were higher than the values found in the subclinically infected and uninfected control dogs. Only clinically affected dogs presented high levels of ALT, FA, GGT and cholesterol in serum. Strong positive correlation was observed between biochemical markers for evaluation of liver damage (ALT, FA, GGT and cholesterol) and hepatic apoptosis (hepatocytes, Kupffer cells, and inflammation). Clinically affected dogs showed a more intense hepatic lesion. Hepatocytes showed a higher rate of apoptosis in Leishmania-infected dogs than in uninfected control dogs. The Kupffer cell apoptotic index and apoptosis within the inflammatory infiltrates were higher in clinically affected dogs. The apoptotic index evaluated in hepatocytes, Kupffer cells, and inflammatory infiltrates showed a positive correlation with the intensity of the hepatic lesion, parasite load, and clinical status. Apoptotic cells also showed positive immunostaining for TUNEL, Bcl2, and Bax. Our data showed that hepatic apoptosis was related to the severity of liver damage, the progression of infection, and the parasite load in leishmaniasis. Apoptotic regulated cell recruitment modulated the inflammatory response and favored the survival and dissemination of parasites, depending on the clinical status of the Leishmania-infected dogs. [Display omitted] •Clinically affected Leishmania-infected dogs showed a more intense hepatic lesion.•The hepatic parasite load was higher in clinically affected dogs.•Hepatocytes showed a higher apoptotic index in Leishmania-infected dogs.•The Kupffer cell apoptotic index was higher in clinically affected dogs.•The inflammatory infiltrate apoptosis was higher in clinically affecte