Cultivation of blue green algae (Arthrospira platensis Gomont, 1892) in wastewater for biodiesel production

• Published in: Chemosphere (Oxford) 2023-09, Vol.335, p.139107-139107, Article 139107
• Main Authors: Salman, Jasim Mohammed, Majrashi, Najwa, Hassan, Fikrat M., Al-Sabri, Ahmed, Abdul-Adel Jabar, Esraa, Ameen, Fuad
• Format: Article
• Language: English
• Subjects: Arthrospira platensis; Biodiesel; Biofuels; Blue green algae; Wastewater
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2023.139107

The production of biodiesel has become an important issue in the effort to reduce gas emissions due to the climate change crisis; therefore, algae have widely used to produce biodiesel for energy sustainability. The present study represented an effort to assess the ability of the alga Arthrospira platensis to produce fatty acids involved in biofuel (diesel) by cultivation in Zarrouk media enriched with different municipal wastewater concentrations. Wastewater was used in different concentrations (5, 15, 25, 35 and 100% [control]). Five fatty acids from the alga were determined and included in the present study. These were inoleic acid, palmitic acid, oleic acid, gamma-linolenic acid, and docosahexaenoic acid. Impact of different cultivation conditions were studied in terms of observed changes in growth rate, doubling time, total carbohydrate, total protein, chlorophyll a, carotenoids, phycocyanin, allophycocyanin, and phycobiliproteins. Results showed an increase in the values of growth rate, total protein content, chlorophyll a, and levels of carotenoids at all treatments except for carbohydrate content, which decreased with an increasing concentration of wastewater. The high value of doubling time (11.605 days) was recorded at treatment 5%. Fatty acids yields were increased at treatment 5% and 15%. The highest concentrations of fatty acids were 3.108 mg/g for oleic acid, gamma-linolenic acid (28.401 mg/g), docosahexaenoic acid (41.707 mg/g), palmitic acid (1.305 mg/g), and linoleic acid (0.296 mg/g). Moreover, the range of phycocyanin (0.017–0.084 mg/l), allophycocyanin (0.023–0.095 mg/l), and phycobiliproteins (0.041–0.180 mg/l) were obtained in treatment with 15–100%, respectively. Cultivation with municipal wastewater reduced the values of nitrate, phosphate, and electrical conductivity as well as increased dissolved oxygen. Maximum electrical conductivity was recorded in untreated wastewater with algae, while the highest level of dissolved oxygen was noted at 35% concentration. The use of the household wastewater is more environmentally friendly as an alternative of the traditional cultivation techniques used for long-term for biofuel production. [Display omitted] •Arthrospira platensis has ability to produce five fatty acids by cultivation in wastewater as alternative media.•The value of nitrate, phosphate were reduced and the dissolved oxygen was increased in 35% concentration.•Increase of biomass, total protein, chlorophyll a and decreased for car