Regulation of innate immunity in marine mussel Mytilus coruscus: MicroRNA Mc-novel_miR_196 targets McTLR-like1 molecule to inhibit inflammatory response and apoptosis

• Published in: Fish & shellfish immunology 2023-07, Vol.138, p.108868-108868, Article 108868
• Main Authors: Wu, Yashu, Si, Xirui, Qiu, Longmei, Chen, Xinglu, Fu, Peipei, Buttino, Isabella, Guo, Baoying, Liao, Zhi, Yan, Xiaojun, Qi, Pengzhi
• Format: Article
• Language: English
• Subjects: Apoptosis; Inflammatory response; microRNA; Mytilus coruscus; Toll-like receptor
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2023.108868

Toll-like receptors (TLRs) are crucial players in immune recognition and regulation, with aberrant activation leading to autoimmune, chronic inflammatory, and infectious diseases. MicroRNAs (miRNAs) have been shown to regulate gene expression at transcriptional and post-transcriptional levels. While miRNA-mediated regulation of TLR signaling has been studied in mammals, the underlying mechanisms of TLR-miRNA interactions in molluscs remain unclear. In a previous study, one of the TLR genes potentially targeted by miRNAs was identified and named McTLR-like1. McTLR-like1 was later found to be targeted by miRNA Mc-novel_miR_196 through bioinformatic prediction. In this study, we aim to experimentally determine the interaction between McTLR-like1 and Mc-novel_miR_196, as well as their functional role in the innate immune response of molluscs. The results showed that the expression of Mc-novel_miR_196 was suppressed, while the expression of McTLR-like1 was enhanced in M. coruscus hemocytes treated with lipopolysaccharide (LPS). Moreover, in vitro assays demonstrated that Mc-novel_miR_196 directly targets the 5′ UTR of McTLR-like1 and leads to the down-regulation of proinflammatory cytokines in hemocytes. In addition, co-transfection experiments confirmed that Mc-novel_miR_196 inhibits McTLR-like1 and inhibits the expression of proinflammatory cytokines. The Tunel assay also showed that Mc-novel_miR_196 inhibited apoptosis in hemocytes induced by LPS. Our findings suggest that microRNA Mc-novel_miR_196 acts as a regulator of innate immunity in M. coruscus by targeting McTLR-like1 and inhibiting inflammatory response and apoptosis. These results provide further insights into the complex molecular mechanisms underlying TLR signaling in molluscs. •Mc-novel_miR_196 was suppressed while McTLR-like1 was enhanced under LPS exposure.•Mc-novel_miR_196 targets the 5′ UTR of McTLR-like1.•Mc-novel_miR_196 inhibits proinflammatory cytokines expression.•Mc-novel_miR_196 inhibites apoptosis in hemocytes induced by LPS.