Novel Use of Cell Profiling Technology to Visualize Mitochondrial Responses of Humpback Whale Fibroblasts to Chemical Exposure

• Published in: Environmental science & technology 2023-06, Vol.57 (24), p.8975-8982
• Main Authors: Hosen, Md Hafiz All, Sykes, Alex M., Wood, Stephen A., Leusch, Frederic D. L., Whitworth, Deanne J., Bengtson Nash, Susan M.
• Format: Article
• Language: English
• Subjects: Animals; Aquatic mammals; Body fat; Cell culture; Chlorpyrifos; Contaminants; Dieldrin; Drug development; Endosulfan; Environmental policy; Exposure; Fibroblasts; Fibroblasts - chemistry; Fibroblasts - metabolism; Food chains; Food webs; Humpback Whale - physiology; Hydrocarbons, Chlorinated - analysis; Hydrocarbons, Chlorinated - metabolism; Lindane; Lipophilic; Mitochondria; Mitochondria - chemistry; Occurrence, Fate, and Transport of Aquatic and Terrestrial Contaminants; Organelles; Pesticides; Pesticides - analysis; Taxa; Trifluralin; Whales
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/acs.est.2c07159

Cetaceans are at elevated risk of accumulating persistent and lipophilic environmental contaminants due to their longevity and high proportion of body fat. Despite this, there is a paucity of taxa-specific chemical effect data, in part due to the ethical and logistical constraints in working with highly mobile aquatic species. Advances in cetacean cell culture have opened the door to the application of mainstream in vitro toxicological effect assessment approaches. Image-based cell profiling is a high-throughput, microscopy-based system commonly applied in drug development. It permits the analysis of the xenobiotic effect on multiple cell organelles simultaneously, hereby flagging its potential utility in the evaluation of chemical toxicodynamics. Here we exposed immortalized humpback whale skin fibroblasts (HuWaTERT) to six priority environmental contaminants known to accumulate in the Southern Ocean food web, in order to explore their subcellular organelle responses. Results revealed chemical-dependent modulation of mitochondrial texture, with the lowest observed effect concentrations for chlorpyrifos, dieldrin, trifluralin, and p,p’-dichlorodiphenyldichloroethane of 0.3, 4.1, 9.3, and 19.8 nM, respectively. By contrast, no significant changes were observed upon exposure to endosulfan and lindane. This study contributes the first fixed mitochondrial images of HuWaTERT and constitutes novel, taxa-specific chemical effect data in support of evidence-based conservation policy and management.