Buffalo sperm membrane glycan-binding proteins reveal precise and preferential binding signatures with specific glycans targets on oviduct epithelium and zona pellucida-an implication in fertilization

• Published in: Theriogenology 2023-09, Vol.207, p.96-109
• Main Authors: Kashyap, Poonam, Solanki, Subhash, Datta, Tirtha Kumar, Kumar, Rakesh
• Format: Article
• Language: English
• Subjects: Animals; Buffaloes - metabolism; Epithelium - metabolism; Female; Female reproductive tract; Fertilization - physiology; Glycans; Lectins - metabolism; Male; Membrane protein; Oviducts - metabolism; Polysaccharides; Semen - metabolism; Sperm-Ovum Interactions; Spermatozoa; Spermatozoa - metabolism; Trisaccharides - metabolism; Trisaccharides - pharmacology; Zona Pellucida - metabolism; Zona Pellucida Glycoproteins
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2023.05.005

Sperm membrane glycan-binding proteins (lectins) interact with the counterpart glycans in the oviduct, oocytes, and vice-versa. It has already been well known that specific glycans are present on oviductal epithelium and zona pellucida (ZP) in different mammalian species. Some of these glycans are necessary for oviductal sperm reservoir formation and gamete recognition. The specific binding phenomenon of lectin-glycans is one of the vital factors for successful fertilization in mammals. We hypothesized that buffalo sperm membrane glycan-binding proteins have specific glycan targets in the oviduct and ZP supporting the fertilization event. In the present investigation, sperm membrane proteins were extracted and assessed for their binding capacity with glycans using a high-throughput glycan microarray. The most promising glycan binding signals were evaluated to confirm the sperm putative receptors for glycan targets in the oviductal epithelial cells (OEC) and on ZP using an in-vitro competitive binding inhibition assay. Based on an array of 100 glycans, we found that N-acetyllactosamine (LacNAc), Lewis-a trisaccharide, 3′-sialyllactosamine and LacdiNAc were the most promising glycans and selected for further in-vitro validation. We established an inhibitory concentration of 12 mM Lewis-a trisaccharide and 10 μg/ml Lotus tetragonolobus (LTL) lectin for the sperm-OEC binding interaction, indicating its specificity and sensitivity. We observed that 3 mM 3′-sialyllactosamine, and LacdiNAc were the most competitive inhibitory concentration in sperm-ZP binding, suggesting a specific and abundance-dependent binding affinity. The competitive binding affinity of Maackia amurensis (MAA) lectin with Neu5Ac(α2-3)Gal(β1-4)GlcNAc further supports the abundance of 3′-sialyllactosamine on ZP responsible for sperm binding. Our findings develop the strong evidence on buffalo sperm putative receptors underlying their locking specificities with Lewis-a trisaccharide in oviduct and 3′-sialyllactosamine on ZP. The functional interaction of buffalo sperm lectins with the target glycans in OEC and ZP appears to be accomplished in an abundance-dependent manner, facilitating the fertilization event in buffaloes. [Display omitted] •The interaction of sperm with the female reproductive tract occurs via the protein-carbohydrate-based mechanism.•The glycan array described the abundances of 100 different glycans on the buffalo sperm surface.•Lewis-a trisaccharide has been identified as a