Studying the Bioactive Components and Phytochemicals of the Methanol Extract of Rhanterium epapposum Oliv

• Published in: Applied biochemistry and biotechnology 2024-05, Vol.196 (5), p.2414-2424
• Main Authors: El-Ashmawy, Ibrahim M., Aljohani, Abdullah S. M., Soliman, Amal S.
• Format: Article
• Language: English
• Subjects: Acids; Asteraceae - chemistry; Benzene; Biochemistry; Biological activity; Biotechnology; Butyric acid; Chemistry; Chemistry and Materials Science; Diabetes mellitus; Dodecanol; Flavonoids; Gas chromatography; Gas Chromatography-Mass Spectrometry; Herbal medicine; Hydrocarbons; Hypertension; Mass spectra; Mass spectrometry; Mass spectroscopy; Methanol; Methanol - chemistry; Ocimene; Original Article; Phenolic compounds; Phenols; Phytochemicals; Phytochemicals - analysis; Phytochemicals - chemistry; Phytochemicals - pharmacology; Plant Components, Aerial - chemistry; Plant Extracts - chemistry; Plant Extracts - pharmacology; Propionic acid; Saponins
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-023-04574-y

Rhanterium epapposum Oliv. (locally known as Al-Arfaj) belongs to the family Asteraceae. This study was designed to discover the bioactive components and phytochemicals of the methanol extract of the aerial parts of Rhanterium epapposum , using Agilent Gas Chromatography-Mass Spectrometry (GC-MS), while the mass spectra of the compounds found in the extract matched with the National Institute of Standards and Technology (NIST08 L). GC-MS analysis of the methanol extract of Rhanterium epapposum aerial parts showed presence of sixteen compounds. The major compounds among these were 9,12,15-octadecatrienoic acid, (Z, Z, Z)- (9.89), n-hexadecenoic acid (8.44), 7-hydroxy-6-methoxy-2H-1-benzopyran-2-one (6.60), benzene propanoic acid, β-amino-4-methoxy- (6.12), 1.4-isopropyl-1,6-dimethyl-1,2,3,4,4 a,7,8,8a-octahedron-1-naphthalenol (6.00), 1-dodecanol, 3,7,11-trimethyl- (5.64), and 9,12-octadecadienoic acid (Z, Z)- (4.84), whereas the minor compounds were 9-Octadecenoic acid, (2-phenyl-1,3-dioxolan-4-yl)methyl ester, trans- (3.63), Butanoic acid (2.93), Stigmasterol (2.92), 2-Naphthalenemethanol (2.66), (2,6,6-Trimethylcyclohex-1-phenylmethanesulfonyl)benzene (2.45), 2-(Ethylenedioxy) ethylamine, N-methyl-N-[4-(1-pyrrolidinyl)-2-butynyl]- (2.00), 1-Heptatriacotanol (1.69), Ocimene (1.59), and β-Sitosterol (1.25). Furthermore, the study was extended to determine the phytochemicals in the methanol extract of Rhanterium epapposum , which indicated the positive presence of saponins, flavonoids, and phenolic compounds. Moreover, quantitative analysis revealed the presence of high content of flavonoids, total phenolic, and tannins. This study outcome suggests a podium of using Rhanterium epapposum aerial parts as a herbal remedy for various diseases especially cancers, hypertension, and diabetes. Graphical Abstract