Electrically conductive 2D-PAN-containing surfaces as a culturing substrate for neurons

In the present contribution we report on a novel route to synthesize 2D-polyaniline (2DPAN) on sulfonated-poly(styrene) (SPS) templates by allowing first monomer assembly followed by chemical oxidation to achieve polymerization. We show that Aplysia neurons grown on 2D-PAN exhibit an unusual growth...

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Bibliographic Details
Published in:Journal of biomaterials science. Polymer ed. 2004-01, Vol.15 (11), p.1355-1374
Main Authors: Oren, R., Sfez, R., Korbakov, N., Shabtai, K., Cohen, A., Erez, H., Dormann, A., Cohen, H., Shappir, J., Spira, M.E., Yitzchaik, S.
Format: Article
Language:English
Subjects:
2D-POLYANILINE (2D-PAN)
Actins - metabolism
Aniline Compounds - chemical synthesis
Aniline Compounds - chemistry
Aniline Compounds - pharmacology
Animals
Aplysia
Biocompatible Materials - chemical synthesis
Biocompatible Materials - chemistry
Biocompatible Materials - pharmacology
Cell Culture Techniques - methods
Cells, Cultured
Computer Simulation
CULTURED APLYSIA NEURONS
Electric Conductivity
MICRO-CONTACT PRINTING (MU CP)
Microscopy, Atomic Force
Microscopy, Confocal
Microscopy, Electron
Microtubules - drug effects
Microtubules - metabolism
Molecular Structure
Neurons - cytology
Neurons - drug effects
Neurons - metabolism
Polylysine - metabolism
SELF-ASSEMBLED MONOLAYERS
Spectrum Analysis
Static Electricity
Sulfur - chemistry
SURFACE MODIFICATIONS
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Summary:In the present contribution we report on a novel route to synthesize 2D-polyaniline (2DPAN) on sulfonated-poly(styrene) (SPS) templates by allowing first monomer assembly followed by chemical oxidation to achieve polymerization. We show that Aplysia neurons grown on 2D-PAN exhibit an unusual growth pattern and adhesion to this conducting substrate that is manifested by the formation of giant lamellipodia. The lamellipodial domains are characterized by small gap between the plasma membrane and the 2D-PAN substrate (ca. 30 nm) and actin rich skeleton resembling the skeleton of growth cones. This behavior is characteristic to uniform substrates containing only 2DPAN. However, in patterned substrates containing additionally poly(L-lysine) Aplysia neurons prefer to extend new neurites on the poly(L-lysine) domains.
ISSN:0920-5063
1568-5624
DOI:10.1163/1568562042368077