Anatomical and molecular characterization of some rhigonematid parasites of millipedes in Nigeria, with new insights into their phylogeny

Parasitic nematodes of millipedes from Nigeria are molecularly characterized for the first time. During nematode surveys on live giant African millipedes from several localities in Nigeria, 4 species of rhigonematids were identified by application of integrative taxonomical approaches (morpho-anatom...

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Bibliographic Details
Published in:Journal of helminthology 2023-06, Vol.97, p.e47-e47, Article e47
Main Authors: Fabiyi, O.A., Bello, T.T., Liébanas, G., Clavero-Camacho, I., Cantalapiedra-Navarrete, C., Archidona-Yuste, A., Palomares-Rius, J.E., Hunt, D.J., Castillo, P.
Format: Article
Language:English
Subjects:
Cytochrome
Cytochromes
Diplopoda
Electron microscopes
Gene sequencing
Genera
Genes
Laboratories
Morphology
Morphometry
Nematodes
Parasites
Phylogenetics
Phylogeny
Research Paper
Ribosomal DNA
rRNA 18S
rRNA 28S
Scanning electron microscopy
Worms
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Summary:Parasitic nematodes of millipedes from Nigeria are molecularly characterized for the first time. During nematode surveys on live giant African millipedes from several localities in Nigeria, 4 species of rhigonematids were identified by application of integrative taxonomical approaches (morpho-anatomy and molecular markers), including Brumptaemilius sp., Gilsonema gabonensis, Obainia pachnephorus, and Rhigonema disparovis. The results of morphometric and molecular analyses of D2-D3 28S, ITS, partial 18S rRNA, and cytochrome oxidase c subunit 1 (COI) gene sequences further characterized the rhigonematid species, and clearly separated them from other related species. Phylogenetic relationships based on 28S and 18S rRNA genes suggest that genera within Ransomnematoidea (Ransomnema, Heth, Carnoya, Brumptaemilius, Cattiena, Insulanema, Gilsonema) and Rhigonematoidea (Rhigonema, Obainia, Xystrognathus, Trachyglossoides, Ichthyocephaloides) clustered rather closer than could be expected in view of their morphological differences. Phylogenetic relationships based on ITS and COI are congruent with those of other ribosomal genes; however, they are not conclusive due to the scarcity of available sequences of these genes for these genera in NCBI.
ISSN:0022-149X
1475-2697
DOI:10.1017/S0022149X23000275