CD8+ T-cell Exhaustion Phenotype in Human Asymptomatic and Ocular Toxoplasmosis

This work analyzed exhaustion markers in CD8+ T-cell subpopulations in 21 samples of peripheral blood mononuclear cells (PBMCs) from individuals with ocular toxoplasmosis (n = 9), chronic asymptomatic toxoplasmosis (n = 7), and non-infected people (n = 5) by using RT-qPCR and flow cytometry techniqu...

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Published in:Ocular immunology and inflammation 2024-09, Vol.ahead-of-print (ahead-of-print), p.1-10
Main Authors: García-López, Laura Lorena, Vargas-Montes, Mónica, Osorio-Méndez, Juan Felipe, Cardona, Néstor, Hernández De Los Ríos, Alejandro, Toro-Acevedo, Carlos Andrés, Arenas-García, Juan Camilo, Mantilla-Muriel, Luz Eliana, Torres, Elizabeth, Valencia-Hernández, Juan D., Acosta-Dávila, Alejandro, de-la-Torre, Alejandra, Celis-Giraldo, Daniel, Mejía Oquendo, Manuela, Sepúlveda-Arias, Juan Carlos, Gómez-Marín, Jorge Enrique
Format: Article
Language:English
Subjects:
Adult
Antigens, CD - genetics
Antigens, CD - metabolism
CD8+ T cells
CD8-Positive T-Lymphocytes - immunology
chronic toxoplasmosis
cytokines
exhaustion markers
Female
Flow Cytometry
Humans
Lymphocyte Activation Gene 3 Protein
Male
Middle Aged
PD-1/PDL-1
Phenotype
Programmed Cell Death 1 Receptor - metabolism
Real-Time Polymerase Chain Reaction
T-Cell Exhaustion
Toxoplasma - immunology
Toxoplasmosis, Ocular - diagnosis
Toxoplasmosis, Ocular - immunology
Toxoplasmosis, Ocular - parasitology
Young Adult
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Summary:This work analyzed exhaustion markers in CD8+ T-cell subpopulations in 21 samples of peripheral blood mononuclear cells (PBMCs) from individuals with ocular toxoplasmosis (n = 9), chronic asymptomatic toxoplasmosis (n = 7), and non-infected people (n = 5) by using RT-qPCR and flow cytometry techniques. The study found that gene expression of PD-1 and CD244, but not LAG-3, was higher in individuals with ocular toxoplasmosis versus individuals with asymptomatic infection or uninfected. Expression of PD1 in CD8+ central memory (CM) cells was higher in nine individuals with toxoplasmosis versus five uninfected individuals (p = .003). After ex vivo stimulation, an inverse correlation was found between the exhaustion markers and quantitative clinical characteristics (lesion size, recurrence index, and number of lesions). A total exhaustion phenotype was found in 55.5% (5/9) of individuals with ocular toxoplasmosis. Our results suggest that the CD8+ exhaustion phenotype is involved in the pathogenesis of ocular toxoplasmosis.
ISSN:0927-3948
1744-5078
1744-5078
DOI:10.1080/09273948.2023.2217906