Hierarchical Mesoporous Metal–Organic Frameworks with Boric Acid Sites on the Inner Surface of Small Mesopores for the Extraction of Nucleotides in Human Plasma Samples

• Published in: ACS applied materials & interfaces 2023-06, Vol.15 (25), p.30643-30652
• Main Authors: Xu, Yidong, Sun, Yao, Yao, Zewei, Wei, Yinmao
• Format: Article
• Language: English
• Subjects: Adsorption; Alcohols; Boric Acids; Chromatography, High Pressure Liquid; Functional Nanostructured Materials (including low-D carbon); Humans; Metal-Organic Frameworks - chemistry; Nucleotides; Organometallic Compounds; Solid Phase Extraction - methods
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.3c05025

In this work, a boronate affinity-functionalized hierarchical mesoporous metal–organic framework adsorbent with boronate sites only in the small mesopore has been structured based on UiO-66@Fe3O4. The introduction of large mesopores in the adsorbent can promote the diffusion of small cis-diol-containing compounds (cis-diols) into small mesopore channels, and the removal of the adsorption sites on the external surface of materials and in large mesopores can enhance the size-exclusion effect of the adsorbent. In addition, the adsorbent has faster adsorption kinetics and excellent selectivity to small cis-diols. Finally, a magnetic dispersive solid-phase extraction coupled with high-performance liquid chromatography was established for the enrichment and detection of nucleotides in plasma. Four nucleotides achieve the recoveries from 93.25 to 118.79%, the limits of detection from 0.35 to 1.26 ng·mL–1, and the intra-day and inter-day relative standard deviations of less than 10.2%. In conclusion, this method can be directly used for the detection of small cis-diol targets in complex biological samples without protein precipitation prior to the extraction.