Effects and regulatory pathway of proopinmelanocortin on feeding habit domestication in mandarin fish

• Published in: Gene 2023-08, Vol.878, p.147581-147581, Article 147581
• Main Authors: Lu, Hai-Lin, Li, Ling, Miao, Yun-Liang, Liang, Hui, Zou, Jia-Ming, You, Jun-Jie, Liang, Xu-Fang, He, Shan
• Format: Article
• Language: English
• Subjects: Animals; ATAC-seq; C-fos; Domestication; Feeding habit domestication; Fish Diseases; Fish Proteins - metabolism; Fishes - metabolism; Habits; Histone methylation; Histones - metabolism; Mandarin fish; Perciformes - genetics; Pro-Opiomelanocortin - genetics; Pro-Opiomelanocortin - metabolism; Proopinmelanocortin; RNA, Messenger - metabolism
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2023.147581

•Pomc played an important role in feeding habits domestication in mandarin fish.•The region with c-fos binding site of pomc gene exhibited positive promoter activity.•The c-fos siRNA treated brain cells displayed suppressed pomc mRNA expression.•c-fos and setd1b were highly expressed in the fish which was difficult to domesticate.•The activation of c-fos gene by histone H3K4me3 was catalyzed by setd1b. Proopiomelanocortin (POMC) is a hormone precursor, and has been reported to participate in domestication. However, its effects on feeding habit domestication in fish are poorly understood. Mandarin fish (Siniperca chuatsi) feeds solely on live prey fish since first-feeding. In the present study, the high expression of pomc in mandarin fish, both the pomc siRNA and MC4R inhibitor treatments increased the success rate of domestication from live prey fish to dead prey fish and food intake of dead prey fish, suggesting the role of pomc on the special feeding habit of live prey fish in mandarin fish. In addition, one c-fos binding site was identified in the region that from −1053 bp to −931 bp upstream of the transcription start site of pomc, and this region exhibited positive promoter activity. The mandarin fish brain cells treated with c-fos siRNA displayed suppressed pomc mRNA expression, indicating that c-fos positively regulated pomc expression. Furthermore, the mRNA expression of c-fos was higher in the mandarin fish which were more difficult to domesticate. The results of ChIP assay and inhibitor treatment confirmed that the activation of c-fos gene by histone H3K4me3 was catalyzed by Setd1b in mandarin fish. Three open peaks were found at the upstream regulatory region of setd1b by ATAC-seq, and the mRNA expression of setd1b was higher in the mandarin fish which were more difficult to domesticate. These results indicated that Setd1b could methylate histone H3K4 to activate the c-fos transcription, maintaining the high expression of pomc, which might contribute to the special feeding habit of mandarin fish.