Ex vivo evaluation of a novel suture loop method for equine intestinal biopsies

Objective To compare a novel suture loop method for intestinal biopsies (SLB) with a two‐layer, hand‐sutured biopsy (HSB) technique in equine small and large intestines. Study design Experimental, randomized, ex vivo study. Animals Eight healthy adult horses. Methods The duodenum, aboral jejunum, an...

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Bibliographic Details
Published in:Veterinary surgery 2024-05, Vol.53 (4), p.723-732
Main Authors: Munsterman, Amelia S., VanderBroek, Ashley R., Kottwitz, Jack J., Watson, Victoria E.
Format: Article
Language:English
Subjects:
Animals
Biopsy
Biopsy - methods
Biopsy - veterinary
Bursting pressure
Diameters
Duodenum
Female
Horses
Intestine
Intestines - pathology
Intestines - surgery
Jejunum
Laparoscopy
Male
Random Allocation
Suture Techniques - veterinary
Sutures
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Summary:Objective To compare a novel suture loop method for intestinal biopsies (SLB) with a two‐layer, hand‐sutured biopsy (HSB) technique in equine small and large intestines. Study design Experimental, randomized, ex vivo study. Animals Eight healthy adult horses. Methods The duodenum, aboral jejunum, and ventral and dorsal large colon were harvested after euthanasia and divided into three sections each. The sections were randomized to SLB, HSB, or control (C, no biopsy) groups. Tissue samples were excised after placement of a suture loop formed by a 4S Roeder knot and oversewn with a Cushing pattern using barbed suture (SLB), or a simple continuous pattern oversewn with a Cushing pattern (HSB). Intraluminal diameter was assessed with contrast radiographs; bursting pressure and wall tension were determined using a solid‐state sensor after instillation of fluid. Tissue samples were evaluated by a board‐certified pathologist. Results Tissue samples were full thickness with similar depth and quality (p > .3). Changes in intraluminal diameter did not differ between methods (p > .16). The bursting pressures were higher for controls than biopsied sections (p 
ISSN:0161-3499
1532-950X
DOI:10.1111/vsu.13983