Bioremediation of Catechol and Concurrent Accumulation of Biocompounds by the Microalga Crypthecodinium cohnii

Burgeoning commercial applications of catechol have led to its excessive accumulation in the environment, thereby posing a severe ecological threat. Bioremediation has emerged as a promising solution. The potential of the microalga Crypthecodinium cohnii to degrade catechol and use the byproduct as...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2023-07, Vol.71 (26), p.10065-10074
Main Authors: Zou, Li-Gong, Yao, Yi-Tong, Wen, Fu-Fang, Zhang, Xiao, Liu, Bo-Tao, Li, Da-Wei, Yang, Yu-Feng, Yang, Wei-Dong, Balamurugan, Srinivasan, Li, Hong-Ye
Format: Article
Language:English
Subjects:
Bioactive Constituents, Metabolites, and Functions
Biodegradation, Environmental
Catechols - metabolism
Dinoflagellida - metabolism
Docosahexaenoic Acids - metabolism
Microalgae - genetics
Microalgae - metabolism
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Summary:Burgeoning commercial applications of catechol have led to its excessive accumulation in the environment, thereby posing a severe ecological threat. Bioremediation has emerged as a promising solution. The potential of the microalga Crypthecodinium cohnii to degrade catechol and use the byproduct as a carbon source was investigated in this study. Catechol significantly increased C. cohnii growth and was rapidly catabolized within 60 h of cultivation. Transcriptomic analysis highlighted the key genes involved in catechol degradation. Real-time polymerase chain reaction (RT-PCR) analysis showed that transcription of key genes CatA, CatB, and SaID involved in the ortho-cleavage pathway was remarkably increased by 2.9-, 4.2-, and 2.4- fold, respectively. Key primary metabolite content was also markedly altered, with a specific increment in polyunsaturated fatty acids. Electron microscopy and antioxidant analysis showed that C. cohnii could tolerate catechol treatment without morphological aberrations or oxidative stress. The findings provide a strategy for C. cohnii in the bioremediation of catechol and concurrent polyunsaturated fatty acids (PUFA) accumulation.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.3c01814