Combination of surfactants and enzyme cocktails for enhancing woody biomass saccharification and bioethanol production from lab-scale to pilot-scale

[Display omitted] •Combinated surfactants increased HTAC pretreated poplar saccharification.•Integration of cellulase cocktail at low dosage 10 FPU/g glucan improved hydrolysis.•The highest glucose titer of 65.3 g/L obtained from 20% solid loading in lab-scale.•Fed-batch SHF of wet pretreated poplar...

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Bibliographic Details
Published in:Bioresource technology 2023-09, Vol.384, p.129343-129343, Article 129343
Main Authors: Qi, Wei, Feng, Qifa, Wang, Wen, Zhang, Yu, Hu, Yunzi, Shakeel, Usama, Xiao, Lin, Wang, Lan, Chen, Hongzhang, Liang, Cuiyi
Format: Article
Language:English
Subjects:
Bioethanol
Biomass
Cellulase enzyme cocktails
Combined surfactants
Ethanol
Fermentation
Hydrolysis
Lignin - chemistry
Pilot-scale operation
Saccharification and fermentation
Surface-Active Agents
Wood - metabolism
Woody biomass
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Summary:[Display omitted] •Combinated surfactants increased HTAC pretreated poplar saccharification.•Integration of cellulase cocktail at low dosage 10 FPU/g glucan improved hydrolysis.•The highest glucose titer of 65.3 g/L obtained from 20% solid loading in lab-scale.•Fed-batch SHF of wet pretreated poplar reached a theoretical ethanol yield of 92.79%.•The ethanol yields of hydrolysate ranged ∼12.48–599.6 kg from pilot-scale operation. Converting woody biomass to bioethanol might be more affordable, environmentally friendly, and efficient for making biofuel commercially feasible, but it would still need a significant optimization process and expand pilot-scale research. A combination of commercial low enzymes loading at 10 FPU/g glucan and compound additives utilizing Tween 80, PEG8000 and sophorolipid applied from lab-scale to pilot-scale have been studied in this work at economically viable dosages for enhancing bioethanol production. In lab-scale saccharification and fermentation, pretreated poplar at a high solid loading of 20% yielded the highest ethanol titers of 30.96 g/L and theoretical ethanol yield of 92.79%. Additionally, pilot-scale operation was used to investigate the bioethanol amplification, a final volume of 33 m3 which yielded the greatest ethanol amount of 599.6 kg from poplar wood while gaining on-site value-added production of hemicellulosic and cellobiose liquor 1122 kg and lignin residues 2292 kg.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2023.129343