Integration of transcriptomic and metabolomic analysis unveils the response mechanism of sugar metabolism in Cyclocarya paliurus seedlings subjected to PEG-induced drought stress

• Published in: Plant physiology and biochemistry 2023-08, Vol.201, p.107856-107856, Article 107856
• Main Authors: Li, Chenhui, Wan, Yifeng, Shang, Xulan, Fang, Shengzuo
• Format: Article
• Language: English
• Subjects: Abiotic stress; Regulatory network; Structural gene; Transcription factors; WGCNA; Wheel wingnut
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2023.107856

Cyclocarya paliurus (Batal.) Iljinskaja is a multiple function tree species used for functional food and valued timber production. Carbohydrates, especially water-soluble carbohydrates, play an important role in osmotic protection, signal transduction and carbon storage. Under the circumstance of global climate change the abiotic stress would restrict the development of C. paliurus plantation, whereas there is few knowledge on the regulatory mechanisms of sugar metabolism under drought stress in C. paliurus. To investigate the drought response of C. paliurus at molecular level, we conducted an integrated analysis of transcriptomic and metabolomic of C. paliurus at three PEG-induced drought stress levels (0%: control; 15%: moderate drought; 25%: severe drought) in short term. Both moderate and severe drought treatments activated the chemical defense with lowering relative water content, and enhancing the contents of soluble protein, proline and malondialdehyde in the leaves. Meanwhile, alterations in the expression of differentially expressed genes and carbohydrate metabolism profiles were observed among the treatments. Weighted gene co-expression network analysis (WGCNA) showed 3 key modules, 8 structural genes (such as genes encoding beta-fructofuranosidase (INV), sucrose synthase (SUS), raffinose synthase (RS)) and 14 regulatory transcription factors were closely linked to sugar metabolism. Our results provided the foundation to understand the response mechanism of sugar metabolism in C. paliurus under drought stress, and would drive progress in breeding of drought-tolerant varieties and plantation development of the species. •Drought stress induced notable reprogramming of transcriptomes in sugar metabolism pathway.•Drought stress resulted in substantial metabolome changes in C. paliurus leaves.•Fourteen transcription factors in sugar metabolism pathway were identified by a regulatory network.•Eight key structural genes were found to be highly correlated with sugar metabolism.