Microorganisms that produce enzymes active on biodegradable polyesters are ubiquitous

• Published in: Biodegradation (Dordrecht) 2023-12, Vol.34 (6), p.489-518
• Main Authors: Degli-Innocenti, Francesco, Breton, Tony, Chinaglia, Selene, Esposito, Ermes, Pecchiari, Marco, Pennacchio, Andrea, Pischedda, Alessandro, Tosin, Maurizio
• Format: Article
• Language: English
• Subjects: Aquatic Pollution; Biodegradability; Biodegradation; Bioinformatics; Biomedical and Life Sciences; Bioplastics; Bonds; Chemical analysis; Decomposers; Depolymerization; Diffusion; Enzymes; Esterases; Esters; Geochemistry; Hydrolases; Hydrolysis; Inoculum; International standards; Life Sciences; Marine sediments; Microbiology; Microorganisms; Plastics; Polyester resins; Polyesters; Polymers; Polyols; Review Paper; Sea-water; Seawater; Sediments; Soil Science & Conservation; Standardization; Terrestrial Pollution; Waste Management/Waste Technology; Waste Water Technology; Water analysis; Water Management; Water Pollution Control
• ISSN: 0923-9820; 1572-9729
• DOI: 10.1007/s10532-023-10031-8

Biodegradability standards measure ultimate biodegradation of polymers by exposing the material under test to a natural microbial inoculum. Available tests developed by the International Organization for Standardization (ISO) use inoculums sampled from different environments e.g. soil, marine sediments, seawater. Understanding whether each inoculum is to be considered as microbially unique or not can be relevant for the interpretation of tests results. In this review, we address this question by consideration of the following: (i) the chemical nature of biodegradable plastics (virtually all biodegradable plastics are polyesters) (ii) the diffusion of ester bonds in nature both in simple molecules and in polymers (ubiquitous); (iii) the diffusion of decomposers capable of producing enzymes, called esterases, which accelerate the hydrolysis of esters, including polyesters (ubiquitous); (iv) the evidence showing that synthetic polyesters can be depolymerized by esterases (large and growing); (v) the evidence showing that these esterases are ubiquitous (growing and confirmed by bioinformatics studies). By combining the relevant available facts it can be concluded that if a certain polyester shows ultimate biodegradation when exposed to a natural inoculum, it can be considered biodegradable and need not be retested using other inoculums. Obviously, if the polymer does not show ultimate biodegradation it must be considered recalcitrant, until proven otherwise.