The microRNA-485-3p concentration in salivary exosome-enriched extracellular vesicles is related to amyloid β deposition in the brain of patients with Alzheimer’s disease

• Published in: Clinical biochemistry 2023-08, Vol.118, p.110603-110603, Article 110603
• Main Authors: Ryu, In Soo, Kim, Dae Hoon, Ro, Ju-Ye, Park, Byeong-Gyu, Kim, Seo Hyun, Im, Jong-Yeop, Lee, Jun-Young, Yoon, Soo Jin, Kang, Heeyoung, Iwatsubo, Takeshi, Teunissen, Charlotte E., Cho, Hyun-Jeong, Ryu, Jin-Hyeob
• Format: Article
• Language: English
• Subjects: Alzheimer’s disease biomarkers; Circulating microRNA; Diagnosis; Neurodegenerative disease; PET studies in Alzheimer’s disease
• ISSN: 0009-9120; 1873-2933
• DOI: 10.1016/j.clinbiochem.2023.110603

•MicroRNA-485-3p concentrations in salivary EE-EVs were increased in the patients with AD vs. those in healthy controls.•MicroRNA-485-3p concentrations from salivary EE-EVs were increased in Aβ-PET-positive AD participants vs. those in Aβ-PET-negative AD participants.•MicroRNA-485-3p concentrations from salivary EE-EVs showed high diagnostic accuracy for predicting Aβ-PET positivity. Alzheimer’s disease (AD) is an irreversible neurodegenerative disease characterized by progressive long-term memory loss and cognitive dysfunction. Neuroimaging tests for abnormal amyloid-β (Aβ) deposition are considered the most reliable methods for the diagnosis of AD; however, the cost for such testing is very high and generally not covered by national insurance systems. Accordingly, it is only recommended for individuals exhibiting clinical symptoms of AD supported by clinical cognitive assessments. Recently, it was suggested that dysregulated microRNA-485-3p (miRNA-485-3p) in the brain and cerebrospinal fluid is closely related to pathogenesis of AD. However, a relationship between circulating miRNA-485-3p in salivary exosome-enriched extracellular vesicles (EE-EV) and Aβ deposition in the brain has not been observed. Using quantitative real-time polymerase chain reaction, we analyzed miRNA-485-3p concentration in salivary EE-EV. We used receiver operating characteristic (ROC) curve analysis to evaluate its predictive value for Aβ positron emission tomography (Aβ-PET) positivity in patients with AD. Our results showed that the miRNA-485-3p concentration in salivary EE-EV isolated from patients with AD was significantly increased compared with that in the healthy controls (p