Transcriptome Analysis of the Spleen Provides Insight into the Immune Regulation of GCRV Resistance in Grass Carp (Ctenopharyngodon idella)

• Published in: Marine biotechnology (New York, N.Y.) N.Y.), 2023-08, Vol.25 (4), p.557-566
• Main Authors: Yu, Chengchen, Wu, Minglin, Jiang, Yuchen, Xu, Xiaoyan, Li, Jiale, Shen, Yubang
• Format: Article
• Language: English
• Subjects: Antigen presentation; Antigen processing; Antigens; Biomedical and Life Sciences; Carp; Chemokines; Control methods; Ctenopharyngodon idella; Cytotoxicity; Down-regulation; Dynamin; Economic importance; Engineering; Fish; Freshwater & Marine Ecology; Freshwater fishes; Gene expression; Genes; Growth factors; Immune system; Immunity; Immunoregulation; Infections; Life Sciences; Ligands; Lymphocytes T; Microbiology; Molecular modelling; Natural killer cells; Nerves; Proteins; Signal transduction; Spleen; Stanniocalcin; Toll-like receptors; Transcriptomes; Transcriptomics; Zoology
• ISSN: 1436-2228; 1436-2236
• DOI: 10.1007/s10126-023-10225-4

Grass carp ( Ctenopharyngodon idella ) is one of the most economically important fish in China, and its production is commonly lost due to GCRV infection. To understand the molecular mechanism of GCRV resistance in grass carp, we compared the spleen transcriptome of the GCRV-resistant and susceptible individuals under GCRV infection (Res-Sus) and the GCRV-resistant individuals under different conditions of injection with GCRV and PBS (Res-Ctl). A total of 87.56 GB of clean data were obtained from 12 transcriptomic libraries of spleen tissues. A total of 379 DEGs (156 upregulated genes and 223 downregulated genes) were identified in the comparison group Res-Ctl. A total of 1207 DEGs (633 upregulated genes and 574 downregulated genes) were identified in the comparison group Res-Sus. And 54 DEGs were shared including immune-related genes of stc2 (stanniocalcin 2), plxna1 (plexin A1), ifnα (interferon alpha), cxcl 11 (C-X-C motif chemokine ligand 11), ngfr (nerve growth factor receptor), mx (MX dynamin-like GTPase), crim1 (cysteine-rich transmembrane BMP regulator 1), plxnb2 (plexin B2), and slit2 (slit guidance ligand 2). KEGG pathway analysis revealed significant differences in the expression of genes mainly involved in immune system and signal transduction, including antigen processing and presentation, Toll-like receptor signaling pathway, natural killer cell-mediated cytotoxicity, and Hippo signaling pathway. This study investigates the immune mechanism of the resistance to GCRV infection in grass carp and provides useful information for the development of methods to control the spread of the GCRV infection.