Application of ATP-based bioluminescence technology in bacterial detection: a review

• Published in: Analyst (London) 2023-07, Vol.148 (15), p.3452-3459
• Main Authors: Liu, Shitong, Zhao, Jinbin, Guo, Yulan, Ma, Xueer, Sun, Chunmeng, Cai, Ming, Chi, Yuyang, Xu, Kun
• Format: Article
• Language: English
• Subjects: Bacteria; Bioluminescence; New technology
• ISSN: 0003-2654; 1364-5528
• DOI: 10.1039/d3an00576c

With the development of new technologies for rapid and high-throughput bacterial detection, ATP-based bioluminescence technology is making progress. Because live bacteria contain ATP, the number of bacteria is correlated with the level of ATP under certain conditions, so that the method of luciferase catalyzing the fluorescence reaction of luciferin with ATP is widely used for the detection of bacteria. This method is easy to operate, has a short detection cycle, does not require much human resources, and is suitable for long-term continuous monitoring. Currently, other methods are being explored in combination with bioluminescence for more accurate, portable and efficient detection. This paper introduces the principle, development and application of bacterial bioluminescence detection based on ATP and compares the combination of bioluminescence and other bacterial detection methods in recent years. In addition, this paper also examines the development prospects and direction of bioluminescence in bacterial detection, hoping to provide a new idea for the application of ATP-based bioluminescence. With the development of new technologies for rapid and high-throughput bacterial detection, ATP-based bioluminescence technology is making progress.