Structural analysis and attenuates hyperuricemic nephropathy of dextran from the Imperata cylindrica Beauv. var. major (Nees) C. E. Hubb

• Published in: Carbohydrate polymers 2023-10, Vol.317, p.121064-121064, Article 121064
• Main Authors: Yu, Wenchen, Xiong, Yi, Liu, Mengnan, Zeng, Deyong, Zhao, Haitian, Liu, Jiaren, Lu, Weihong
• Format: Article
• Language: English
• Subjects: Animals; Dextrans - metabolism; Hyperuricemic nephropathy; Imperata cylindrica; Kidney - metabolism; Mice; NF-kappa B - metabolism; NMR spectrometry; Phosphatidylinositol 3-Kinases - metabolism; Poaceae - metabolism; Uric Acid
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2023.121064

ICPC-a was from the Imperata cylindrica with a molecular weight of 45 kDa, which was composed of α-D-1,3-Glcp and α-D-1,6-Glcp. The ICPC-a showed thermal stability, maintaining its structural integrity up to 220°C. X-ray diffraction analysis confirmed its amorphous nature, while scanning electron microscopy revealed a layered morphology. ICPC-a significantly ameliorated uric acid stimulation-induced HK-2 cell injury and apoptosis and reduced uric acid levels in mice with hyperuricemic nephropathy. ICPC-a protected against renal injury by inhibiting lipid peroxidation levels, increasing antioxidant damage and defense levels, inhibiting secretion of pro-inflammatory factors, regulating purine metabolism, PI3K-Akt signaling pathway, NF-κB signaling pathway, inflammatory bowel disease, mTOR signaling pathway, and MAPK signaling pathway. These findings indicate that ICPC-a is a promising natural substance with multiple targets, multiple pathways of action, and without toxicity, making it a valuable subject for further research and development. [Display omitted]