Culture of cerebrospinal fluid-contacting neurons from neonatal mouse spinal cord

Cerebrospinal fluid-contacting neurons (CSF-cNs) act crucial role in chemosensory and mechanosensory function in spinal cord. Recently, CSF-cNs were found to be an immature neuron and may be involved in spinal cord injury recovery. But how to culture it and explore its function in vitro are not repo...

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Bibliographic Details
Published in:Cell and tissue banking 2024-06, Vol.25 (2), p.443-452
Main Authors: Cao, Liang, Zhang, Hui-Qian, He, Yu-Qi, An, Ping-Jiang, Yang, Lei-Luo, Tan, Wei, Liu, Gang, Wang, Chun-Qing, Dou, Xiao-Wei, Li, Qing
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Biomarkers - cerebrospinal fluid
Biomarkers - metabolism
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell culture
Cell Culture Techniques
Cells, Cultured
Cerebrospinal fluid
Cerebrospinal Fluid - cytology
Cerebrospinal Fluid - metabolism
Chemoreception
Flow cytometry
Full Length Paper
Glial fibrillary acidic protein
Hospitals
Kidney diseases
Life Sciences
Mice
Morphology
Neonates
Nestin
Neural stem cells
Neural Stem Cells - cytology
Neural Stem Cells - metabolism
Neurons
Neurons - cytology
Neurons - metabolism
Orthopedics
Penicillin
Polycystic kidney
Spinal cord
Spinal Cord - cytology
Spinal cord injuries
Stem cells
Transplant Surgery
Tubulin
γ-Aminobutyric acid
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Summary:Cerebrospinal fluid-contacting neurons (CSF-cNs) act crucial role in chemosensory and mechanosensory function in spinal cord. Recently, CSF-cNs were found to be an immature neuron and may be involved in spinal cord injury recovery. But how to culture it and explore its function in vitro are not reported in previous research. Here, we first reported culture and identification of CSF-cNs in vitro. We first established a protocol for in vitro culture of CSF-cNs from the cervical spinal cord of mice within 24 h after birth. Polycystic kidney disease 2-like 1 (PKD2L1) + cells were isolated by fluorescence-activated cell sorting and expressed the neuron marker β-tubulin III and CSF-cNs marker GABA. Intriguingly, PKD2L1 + cells formed neurosphere and expressed neural stem cell markers Nestin, Sox2 and GFAP. Thus, our research provided culture and isolation of CSF-cNs and this facilitate the investigation the CSF-cNs function in vitro.
ISSN:1389-9333
1573-6814
DOI:10.1007/s10561-023-10098-w