Protective effect of melatonin against oxygen-induced retinopathy: a study based on the HMGB1/NF-κB/NLRP3 axis

• Published in: Zhongguo dang dai er ke za zhi 2023-06, Vol.25 (6), p.645-652
• Main Authors: Chu, Fang-Fang, Zhao, Yan-Song, Zhao, Yu-Ze, Bai, Chen, Xiao, Pei-Lun, Wang, Xiao-Li, Yu, Shu-Na, Jiang, Ji-Ying
• Format: Article
• Language: Chinese
• Subjects: Animals; HMGB1 Protein; Melatonin - pharmacology; Melatonin - therapeutic use; Mice; Mice, Inbred C57BL; NF-kappa B; NLR Family, Pyrin Domain-Containing 3 Protein; Oxygen - adverse effects; Peroxidase; Receptors, Melatonin; Retinal Diseases - chemically induced; Retinal Diseases - drug therapy
• ISSN: 1008-8830
• DOI: 10.7499/j.issn.1008-8830.2301036

To study the protective effect of melatonin (Mel) against oxygen-induced retinopathy (OIR) in neonatal mice and the role of the HMGB1/NF-κB/NLRP3 axis. Neonatal C57BL/6J mice, aged 7 days, were randomly divided into a control group, a model group (OIR group), and a Mel treatment group (OIR+Mel group), with 9 mice in each group. The hyperoxia induction method was used to establish a model of OIR. Hematoxylin and eosin staining and retinal flat-mount preparation were used to observe retinal structure and neovascularization. Immunofluorescent staining was used to measure the expression of proteins and inflammatory factors associated with the HMGB1/NF-κB/NLRP3 axis and lymphocyte antigen 6G. Colorimetry was used to measure the activity of myeloperoxidase. The OIR group had destruction of retinal structure with a large perfusion-free area and neovascularization, while the OIR+Mel group had improvement in destruction of retinal structure with reductions in neovascularization and perfusion-free area. Compared with t