Role and mechanism of platelet-derived growth factor BB in thrombocytosis in Kawasaki disease

To study the role and mechanism of platelet-derived growth factor BB (PDGF-BB) on platelet production in Kawasaki disease (KD) mice and human megakaryocytic Dami cells through and experiments. ELISA was used to measure the expression of PDGF in the serum of 40 children with KD and 40 healthy childre...

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Bibliographic Details
Published in:Zhongguo dang dai er ke za zhi 2023-06, Vol.25 (6), p.579-586
Main Authors: Shen, Xi-Wei, Tang, Zhi-Yuan, Shen, Xian-Juan, Zhao, Jian-Mei
Format: Article
Language:Chinese
Subjects:
Animals
Becaplermin
Child
Humans
Imatinib Mesylate - pharmacology
Imatinib Mesylate - therapeutic use
Mice
Mice, Inbred C57BL
Mucocutaneous Lymph Node Syndrome - drug therapy
Phosphatidylinositol 3-Kinases
Proto-Oncogene Proteins c-akt
RNA, Messenger
Thrombocytosis - drug therapy
Thrombocytosis - etiology
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Summary:To study the role and mechanism of platelet-derived growth factor BB (PDGF-BB) on platelet production in Kawasaki disease (KD) mice and human megakaryocytic Dami cells through and experiments. ELISA was used to measure the expression of PDGF in the serum of 40 children with KD and 40 healthy children. C57BL/6 mice were used to establish a model of KD and were then randomly divided into a normal group, a KD group, and an imatinib group (30 mice in each group). Routine blood test was performed for each group, and the expression of PDGF-BB, megakaryocyte colony forming unit (CFU-MK), and the megakaryocyte marker CD41 were measured. CCK-8, flow cytometry, quantitative real-time PCR, and Western blot were used to analyze the role and mechanism of PDGF-BB in platelet production in Dami cells. PDGF-BB was highly expressed in the serum of KD children (
ISSN:1008-8830
DOI:10.7499/j.issn.1008-8830.2301086