Engineered Collaborative Size Regulation and Shape Engineering of Tremella‐Like Au‐MnOx for Highly Sensitive Bimodal‐Type Lateral Flow Immunoassays

• Published in: Small (Weinheim an der Bergstrasse, Germany) Germany), 2023-10, Vol.19 (43), p.e2301598-n/a
• Main Authors: Liu, Sijie, Liao, Yangjun, Zhang, Yinuo, Shu, Rui, Zhang, Mingrui, Luo, Xing, Sun, Chenyang, Dou, Leina, Luo, Linpin, Sun, Jing, Zhang, Daohong, Zeng, Lintao, Wang, Jianlong
• Format: Article
• Language: English
• Subjects: Ambient temperature; Anisotropy; Antibodies; Assaying; bimodal‐type; Business competition; Collaboration; Colorimetry; Functional materials; Gold; Immunoassay; Immunology; lateral flow immunoassays; Nanomaterials; Nanoparticles; Nanotechnology; photothermal; Redox reactions; shape engineering; Signal transduction; size regulation
• ISSN: 1613-6810; 1613-6829
• DOI: 10.1002/smll.202301598

Engineered collaborative size regulation and shape engineering of multi‐functional nanomaterials (NPs) offer extraordinary opportunities for improving the analysis performance. It is anticipated to address the difficulty in distinguishing color changes caused by subtle variations in target concentrations, thereby facilitating the highly sensitive analysis of lateral flow immunoassays (LFIAs). Herein, tremella‐like gold‐manganese oxide (Au‐MnOx) nanoparticles with precise MnCl2 regulation are synthesized as immuno signal tracers via a facile one‐step redox reaction in alkaline condition at ambient temperature. Avail of the tunable elemental composition and anisotropy in morphology, black‐colored tremella‐like Au‐MnOx exhibits superb colorimetric signal brightness, enhanced antibody coupling efficiency, marvelous photothermal performance, and unrestricted immunological recognition affinity, all of which facilitate highly sensitive multi‐signal transduction patterns. In conjunction with the handheld thermal reader device, a bimodal‐type LFIA that combines size‐regulation‐ and shape‐engineering‐mediated colorimetric‐photothermal dual‐response assay (coined as the SSCPD assay) with a limit of detection of 0.012 ng mL−1 for ractopamine (RAC) monitoring is achieved by integrating Au‐MnOx with the competitive‐type immunoreaction. This work illustrates the effectiveness of this strategy for establishing high‐performance sensing, and the SSCPD assay may be extended to a wide spectrum of future point‐of‐care (POC) diagnostic applications. To circumvent the issue of difficulty in distinguishing color changes due to subtle variations in target concentrations and facilitate high‐sensitivity analysis of lateral flow immunoassays (LFIAs), engineered collaborative size regulationand shape engineering supported black tremella‐like gold‐manganese oxide (Au‐MnOx) nanoparticles with precise MnCl2 regulation are prepared for bimodal‐type LFIA via a facile one‐pot redox reaction in an alkaline environment.