Alu Retrotransposition Event in SPAST Gene as a Novel Cause of Hereditary Spastic Paraplegia

To diagnose the molecular cause of hereditary spastic paraplegia (HSP) observed in a four-generation family with autosomal dominant inheritance. Multiplex ligation-dependent probe amplification (MLPA), whole-exome sequencing (WES), and RNA sequencing (RNA-seq) of peripheral blood leukocytes were per...

Full description

Saved in:
Bibliographic Details
Published in:Movement disorders 2023-09, Vol.38 (9), p.1750-1755
Main Authors: Chen, Yi-Jun, Wang, Meng-Wen, Qiu, Yu-Sen, Yuan, Ru-Ying, Wang, Ning, Lin, Xiang, Chen, Wan-Jin
Format: Article
Language:English
Subjects:
Autosomal dominant inheritance
Hereditary spastic paraplegia
Leukocytes
Movement disorders
Paralysis
Peripheral blood
Phenotypes
Polymerase chain reaction
Retrotransposition
Reverse transcription
Ribonucleic acid
RNA
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To diagnose the molecular cause of hereditary spastic paraplegia (HSP) observed in a four-generation family with autosomal dominant inheritance. Multiplex ligation-dependent probe amplification (MLPA), whole-exome sequencing (WES), and RNA sequencing (RNA-seq) of peripheral blood leukocytes were performed. Reverse transcription polymerase chain reaction (RT-PCR) and Sanger sequencing were used to characterize target regions of SPAST. A 121-bp AluYb9 insertion with a 30-bp poly-A tail flanked by 15-bp direct repeats on both sides was identified in the edge of intron 16 in SPAST that segregated with the disease phenotype. We identified an intronic AluYb9 insertion inducing splicing alteration in SPAST causing pure HSP phenotype that was not detected by routine WES analysis. Our findings suggest RNA-seq is a recommended implementation for undiagnosed cases by first-line diagnostic approaches. © 2023 International Parkinson and Movement Disorder Society.
ISSN:0885-3185
1531-8257
DOI:10.1002/mds.29522