Anti-biofilm effect of salivary histatin 5 on Porphyromonas gingivalis

• Published in: Applied microbiology and biotechnology 2023-08, Vol.107 (16), p.5179-5189
• Main Authors: Li, Rui, Hou, Mengjie, Yu, Liying, Luo, Wen, Kong, Jie, Yu, Renmei, Liu, Ruihan, Li, Qian, Tan, Lisi, Pan, Chunling, Wang, Hongyan
• Format: Article
• Language: English
• Subjects: Alveolar bone; Antimicrobial peptides; Biofilms; Biomedical and Life Sciences; Biotechnology; Bone loss; Bone resorption; Care and treatment; Confocal microscopy; Genomics; Gentian violet; Gram-negative bacteria; Gum disease; Identification and classification; Inflammation; Laser scanning microscopy; Life Sciences; Membrane proteins; Membranes; Metabolism; Microbial Genetics and Genomics; Microbiology; Microscopy; Periodontitis; Polymerase chain reaction; Porphyromonas gingivalis; Properties; Proteins; Proteomics; Scanning electron microscopy; Scanning microscopy; Transcriptomics
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-023-12664-4

This study aimed to investigate the effects of salivary histatin 5 (Hst5) on Porphyromonas gingivalis ( P. gingivalis ) biofilms in vitro and in vivo and the possible mechanisms. In in vitro experiments, P. gingivalis biomass was determined by crystal violet staining. Polymerase chain reaction, scanning electron microscopy, and confocal laser scanning microscopy were used to determine the Hst5 concentration. A search for potential targets was performed using transcriptomic and proteomic analyses. In vivo experimental periodontitis was established in rats to evaluate the effects of Hst5 on periodontal tissues. Experimental results showed that 25 µg/mL Hst5 effectively inhibited biofilm formation, and increased concentrations of Hst5 increased the inhibitive effect. Hst5 might bind to the outer membrane protein RagAB. A combination of transcriptomic and proteomic analyses revealed that Hst5 could regulate membrane function and metabolic processes in P. gingivalis , in which RpoD and FeoB proteins were involved. In the rat periodontitis model, alveolar bone resorption and inflammation levels in periodontal tissues were reduced by 100 µg/mL Hst5. This study showed that 25 µg/mL Hst5 inhibited P. gingivalis biofilm formation in vitro by changing membrane function and metabolic process, and RpoD and FeoB proteins might play important roles in this process. Moreover, 100 µg/mL Hst5 inhibited periodontal inflammation and alveolar bone loss in rat periodontitis via its antibacterial and anti-inflammatory effects. Key points • Anti-biofilm activity of histatin 5 on Porphyromonas gingivalis was investigated . • Histatin 5 inhibited Porphyromonas gingivalis biofilm formation . • Histatin 5 showed inhibitory effects on the occurrence of rat periodontitis .