Assessment of the stallion sperm acrosome in two different extenders: Spermac stain in comparison with PNA/PSA/PI triple-staining

Since the stallion acrosome is very small compared to other species and cannot be properly assessed without additional staining, several labelling techniques were developed to facilitate its assessment. The aim of this study was to compare the Spermac stain (Minitüb GmbH) and a PNA/PSA/PI triple-sta...

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Bibliographic Details
Published in:Reproduction in domestic animals 2023-09, Vol.58 (9), p.1330-1333
Main Authors: Dierberger, H, Pieper, L, Jung, M, Schulze, M
Format: Article
Language:English
Subjects:
Acrosomes
Correlation coefficient
Correlation coefficients
Flow cytometry
Labeling
Semen
Sperm
Staining
Stains
Stains & staining
Yolk
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Summary:Since the stallion acrosome is very small compared to other species and cannot be properly assessed without additional staining, several labelling techniques were developed to facilitate its assessment. The aim of this study was to compare the Spermac stain (Minitüb GmbH) and a PNA/PSA/PI triple-staining detected by flow cytometry with regard to method agreement for detecting non-intact acrosomes within two different extenders. For this purpose, eighteen stallion ejaculates were split in half and diluted with the semen extenders EquiPlus or Gent (Minitüb GmbH) to a final concentration of 50 × 10  sperm/mL, respectively. Subsequently, 126 semen samples were stained with both methods between 4 and 240 h (mean: 63.8 ± 48.9 h) after semen collection. Calculated Intraclass correlation coefficients revealed excellent correlations between both methods for EquiPlus (r = .77, p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.14426