Molecular characterization of a novel ambiguivirus isolated from the phytopathogenic fungus Setosphaeria turcica

In this study, a novel single-stranded RNA virus was isolated from the plant-pathogenic fungus Setosphaeria turcica strain TG2, and the virus was named "Setosphaeria turcica ambiguivirus 2" (StAV2). The complete nucleotide sequence of the StAV2 genome was determined using RT-PCR and RLM-RA...

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Bibliographic Details
Published in:Archives of virology 2023-08, Vol.168 (8), p.199-199, Article 199
Main Authors: Jia, Jichun, Chen, Xu, Wang, Xue, Liu, Xu, Zhang, Nuo, Zhang, Baojun, Chang, Yindong, Mu, Fan
Format: Article
Language:English
Subjects:
Ambiguiviridae
Amino acid sequence
Annotated Sequence Record
Biomedical and Life Sciences
Biomedicine
Fusion protein
Genomes
Infectious Diseases
Medical Microbiology
Nucleotide sequence
Open reading frames
Phylogeny
Phytopathogenic fungi
Proteins
RNA viruses
Setosphaeria turcica
Stop codon
Virology
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Summary:In this study, a novel single-stranded RNA virus was isolated from the plant-pathogenic fungus Setosphaeria turcica strain TG2, and the virus was named "Setosphaeria turcica ambiguivirus 2" (StAV2). The complete nucleotide sequence of the StAV2 genome was determined using RT-PCR and RLM-RACE. The StAV2 genome comprises 3,000 nucleotides with a G+C content of 57.77%. StAV2 contains two in-frame open reading frames (ORFs) with the potential to produce an ORF1-ORF2 fusion protein via a stop codon readthrough mechanism. ORF1 encodes a hypothetical protein (HP) of unknown function. The ORF2-encoded protein shows a high degree of sequence similarity to the RNA-dependent RNA polymerases (RdRps) of ambiguiviruses. BLASTp searches showed that the StAV2 HP and RdRp share the highest amino acid sequence identity (46.38% and 69.23%, respectively) with the corresponding proteins of a virus identified as " Riboviria sp." isolated from a soil sample. Multiple sequence alignments and phylogenetic analysis based on the amino acid sequences of the RdRp revealed that StAV2 is a new member of the proposed family " Ambiguiviridae ".
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-023-05829-z