The effect of a preparation containing glycocholic acid on the biocidal efficacy of sodium hypochlorite in a biofilm laboratory model

• Published in: Australian endodontic journal 2023-12, Vol.49 (3), p.503-511
• Main Authors: Haddad, Yoseif, Chan, Wing Chuan, Ha, William Nguyen, Conde, Antonio, Estevez, Roberto, Lopez, Alejandro Peña, Gates, Georgia Charlotte, Rossi‐Fedele, Giampiero
• Format: Article
• Language: English
• Subjects: Anti-Infective Agents - pharmacology; Antimicrobial activity; anti‐infective agents; Biofilms; Dental Pulp Cavity; Endodontics; Enterococcus faecalis; glycocholic acid; Glycocholic Acid - pharmacology; Hypochlorous acid; Hypochlorous Acid - pharmacology; root canal irrigants; Root Canal Irrigants - pharmacology; Root Canal Preparation - methods; Root canals; Sodium hypochlorite; Sodium Hypochlorite - pharmacology
• ISSN: 1329-1947; 1747-4477
• DOI: 10.1111/aej.12771

This study assessed the antimicrobial effect of sodium hypochlorite (NaOCl) mixtures combined with Keratobacter (KB) using an engineered biofilm root canal model. Clinical and reagent grade NaOCl were mixed with KB (9:1‐vol/vol) to assess pH values over 1 min to select the ideal solution with a pH just below the pKa of hypochlorous acid. The samples were randomly divided into five groups: 1% and 4% NaOCl reagents, a mixture of NaOCl:KB using 1% and 4% NaOCl reagents and distilled water. Outcome measures were colony‐forming units (CFUs/mL) and positive/negative cultures. No significant differences were observed in the pairwise comparisons between 1%, 4% NaOCl and 4% NaOCl+KB for the outcome CFUs/mL. Only 4% NaOCl presented with negative cultures in all samples, whereas 1% NaOCl and 4% NaOCl+KB had similar results (54% vs. 40%). The addition of KB has a limited effect on the antimicrobial efficacy of 4% NaOCl in this laboratory model.