Expanding the use of portable XRF to monitor lead exposure in an Australian duck species two decades after a ban on lead shot

• Published in: The Science of the total environment 2023-04, Vol.869, p.161803, Article 161803
• Main Authors: Nzabanita, Damien, Hampton, Jordan O., Toop, Simon D., Bengsen, Andrew J., Specht, Aaron J., Flesch, Jason S., Hufschmid, Jasmin, Nugegoda, Dayanthi
• Format: Article
• Language: English
• Subjects: Animals; atomic absorption spectrometry; Australia; Avian; Biomonitoring; Birds; Ducks; Ecotoxicology; environment; Environmental monitoring; Fluorescence; Humans; humerus; Hunting; Lead; Lead - analysis; Lead Poisoning - veterinary; regression analysis; species; toxicity; Victoria; waterfowl; wildlife; Wildlife management; X-radiation
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2023.161803

There is growing worldwide recognition of the threat posed by toxic lead for wildlife and humans. Lead toxicity from ammunition has been shown to be a threat to waterbirds across the globe. Lead shot was banned for all waterfowl hunting in Victoria, Australia, in 2002. However, no assessments of lead exposure in Australian waterfowl have been published since the 1990s. Our aim was to estimate contemporary lead exposure via measuring bone lead concentrations in a harvested dabbling duck, the Pacific black duck (Anas superciliosa). We collected wings from 77 Pacific black ducks, spanning 2018 (n = 30) and 2021 (n = 47), from nine sites with long-term histories of regular waterfowl hunting. We sought to validate portable X-ray fluorescence (XRF) for this purpose by taking a piece of humerus bone from each bird, and measuring lead concentration (mg/kg), first via non-destructive XRF and then via destructive inductively coupled plasma mass spectrometry (ICP-MS) and validated the relationship via regression analysis. Portable XRF bone lead measurement demonstrated a strong correlation with ICP-MS results using root-transformed regression (R2 = 0.85). Greater than 92 % of ducks had only background lead exposure (20 mg/kg) ∼3-fold lower (2.6 c.f. 7.5 %). Our results confirm that portable XRF is a useful option for measurement of bone lead in Australasian waterbird species. Our findings also demonstrate that a ban on the use of lead shot around 20 years ago has been associated with a substantial reduction in lead exposure in at least one species of waterfowl. [Display omitted] •Lead exposure threatens waterbirds globally, but is under-recognized in Australia.•Bone lead was measured in Pacific black ducks ∼20 years after a ban on lead shot.•Portable X-ray fluorescence was validated for measuring bone lead levels.•Lead levels were reduced since the last published study >30 years ago.•XRF data were strongly correlation with digestion-based lead concentration data.