Increased lentivirus titer using ultra expression vectors

Lentivirus is an efficient gene transfer system that is widely used in basic science. We aimed to improve viral titer by applying an ultra-expression vectors to lentiviral packaging. Application of the ultra-expression vectors increased biological titer 4 times for standard preparation. We also eval...

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Bibliographic Details
Published in:Analytical biochemistry 2023-05, Vol.669, p.115119-115119, Article 115119
Main Authors: Fujibayashi, So, Kiyono, Tohru, Endo, Yuka, Tani, Tetsuya, Tate, Haruka, Bai, Lanlan, Sugano, Eriko, Tomita, Hiroshi, Fukuda, Tomokazu
Format: Article
Language:English
Subjects:
Base Sequence
biochemistry
gene transfer
Genetic Vectors - genetics
Lentivirus
Lentivirus - genetics
Lentivirus - metabolism
Packaging system
Transcriptional factors
Transduction, Genetic
Ultra-expression vectors
viral load
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Summary:Lentivirus is an efficient gene transfer system that is widely used in basic science. We aimed to improve viral titer by applying an ultra-expression vectors to lentiviral packaging. Application of the ultra-expression vectors increased biological titer 4 times for standard preparation. We also evaluated the efficacy of the ultra-expression vectors to relatively longer insert fragments, such as CSII-CMV-CNROE containing 5 genes in multiple cloning sites. Packaging of the ultra-expression vectors showed 3.5 times higher biological titer compared with the original method. Our improved packaging system could be applied to lentivirus to produce higher titers. [Display omitted] •Application of the ultra-expression vectors increased biological titer of lentivirus around 4 times for standard preparation.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2023.115119