Propagation and immunological characterization of coxsackievirus A10 in a serum-free HEK293A cell culture system

• Published in: Virus research 2023-05, Vol.329, p.199101-199101, Article 199101
• Main Authors: Lien, Sheng-Chieh, Shen, Yu-Sheng, Lin, Hsiao-Yu, Wu, Shang-Rung, Fang, Chih-Yeu, Chen, Chi-Hsun, Chen, Yi-An, Chong, Pele Choi-Sing, Huang, Ming-Hsi, Chow, Yen-Hung, Wang, Jen-Ren, Wu, Suh-Chin, Liu, Chia-Chyi
• Format: Article
• Language: English
• Subjects: Animals; antibodies; Antibodies, Neutralizing; Antibodies, Viral; cell culture; Coxsackievirus A10 (CVA10); Enterovirus; Enterovirus A; Enterovirus A, Human - genetics; Hand foot and mouth diseases; Hand, Foot and Mouth Disease - prevention & control; HEK293A; immunogenicity; Inactivated whole virion vaccine; manufacturing; Mice; mouth; Serum-free cell culture; ultracentrifugation; Vaccines, Inactivated; viral vaccines; viruses
• ISSN: 0168-1702; 1872-7492
• DOI: 10.1016/j.virusres.2023.199101

•Many coxsackievirus A10 isolates have low culture efficiency in vero cells.•HEK293A cell is a suitable cell line for coxsackievirus A10 propagation.•A serum-free HEK293A cell culture was developed for coxsackievirus A10 production.•Other enteroviruses could also be propagated in serum-free HEK293A cell culture. Coxsackievirus A10 (CVA10) is one of enteroviral pathogens that cause the hand, foot, and mouth disease (HFMD). Since CVA10 was reported to be not easily propagated in the Vero cell culture, a feasible manufacture process for producing formalin-inactivated CVA10 vaccine is urgently needed. Several cell lines that commonly used for viral vaccine production was tested for CVA10 (M2014 strain) culture in this study, and our result showed that CVA10 could be easily propagated in the HEK293A cells. A serum-free HEK293A cell culture system was developed for CVA10 production and the yields have reached over 108 TCID50/mL. The biochemical and immunogenic properties of CVA10 particles obtained from this serum-free HEK293A culture were identical to our previous study. Two major particles of CVA10 were separated by ultracentrifugation, and only the infectious mature particles were capable of inducing CVA10 neutralizing antibody responses in the mouse immunogenicity studies. Additionally, we found that coxsackievirus A6 and enterovirus A71 could also be easily propagated using this serum-free HEK293A cell culture system. Our results provide a solution to overcome the obstacle in the propagation of CVA10 and facilitate the development of multivalent vaccines for prevention of HFMD.