Pingyin Rose Essential Oil Restores Intestinal Barrier Integrity in DSS-induced Mice Colitis Model

• Published in: Food research international 2023-02, Vol.164, p.112362-112362, Article 112362
• Main Authors: Raka, Rifat Nowshin, Xiao, Junsong, Wu, Hua, Lv, Wenwen, Ding, Zhiqian, Cao, Yangping, Li, Xukai, Sun, Jinglong, Luan, Kou
• Format: Article
• Language: English
• Subjects: Animals; Anti-inflammation; blood; catalase; China; colitis; Colitis - chemically induced; Colitis - drug therapy; Colitis - metabolism; colon; dextran; Dextran Sulfate - adverse effects; drugs; essential oils; Female; females; food additives; food industry; food research; functional foods; inflammation; interleukin-6; intestinal microorganisms; Intestinal permeability; malondialdehyde; Mice; myeloperoxidase; NF-kappa B - metabolism; nitric oxide; Oils, Volatile - adverse effects; Oxidative stress; Rosa; Rosa rugosa; Rose essential oil; smell; sodium sulfate; superoxide dismutase; tight junctions; TLR4-NF-κB signaling pathway; transcriptomics; Tumor Necrosis Factor-alpha; tumor necrosis factors; weight loss; Western blotting
• ISSN: 0963-9969; 1873-7145
• DOI: 10.1016/j.foodres.2022.112362

[Display omitted] •PREO restores intestinal barrier integrity.•PREO promotes tight junction (TJ) protein ZO-1, Occludin and mucin protein MUC2 expressions.•PREO modulates oxidative and inflammatory stress.•PREO recovers the mucosal injury through the TLR4-NF-κB pathway. Rosa rugosa cv. Plena is a ‘drug homologous food’ in China with a long history. Pingyin rose essential oil (PREO) is a mixture of compounds extracted from blooming R. rugosa cv. Plena. With its elegant smell and excellent effects on oxidative stress and inflammation alleviation, PREO is wildly used in the food industry as a popular additive. We aimed to decipher if the PREO could alleviate and restore dextran sodium sulfate (DSS)-induced barrier integrity damages. The results showed that a 7-day PREO (15 μL/kg) treatment alleviated the colitis symptoms by improving disease activity index (DAI) scores through weight loss, occult blood, and colon shortening. The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated BALB/c female mice. PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6. Further, PREO modulated the composition of the gut microbiota and Spearman’s correlation analysis revealed a positive effect. The transcriptome analysis and western blot results indicated that PREO might ameliorate intestinal barrier dysfunction in this study via the TLR4-NF-kB signaling pathway. We hypothesized that PREO has preventive potential against gut disorders and could serve as a functional food additive.