Antineoplastic effects of cassava-cyanide extract on human glioblastoma (LN229) cells

• Published in: Toxicon (Oxford) 2023-08, Vol.232, p.107200-107200, Article 107200
• Main Authors: S, Sreejith, Tom, Joseph, V P, Sangeetha, U, Vandana, Xavier, Joseph, C A, Jayaprakas, P V, Mohanan
• Format: Article
• Language: English
• Subjects: Cassava cyanogenic glycosides; Cytotoxicity; HCN; Linamarin; LN229
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/j.toxicon.2023.107200

Several natural compounds reduce tumour cell growth and metastasis by inducing programmed cell death. Cassava (Manihot esculenta Crantz) contains cyanogenic glycosides such as, linamarin and lotaustralin, can be enzymatically cleaved by linamarase to release hydrogen cyanide (HCN), which can have therapeutic benefits against hypertension, asthma, and cancer. We have developed a technology for isolating bio-active principles from cassava leaves.The present study is designed to analyze the cytotoxic effect of cassava cyanide extract (CCE) against human glioblastoma cells (LN229). The treatment of CCE demonstrated a dose dependent toxicity on glioblastoma cells. At higher concentration tested, the CCE (400 μg/mL) was found to be cytotoxic, reducing the cell viability to 14.07 ± 2.15% by negatively influencing the mitochondrial activity, and lysosomal and cytoskeletal integrity. Coomassie's brilliant blue staining confirmed cells' morphological aberration after 24 h of treatment with CCE. Moreover, DCFH-DA assay and Griess reagent showed an increase in ROS but a decrease in RNS production at a concentration of CCE. Flow cytometry analysis revealed that CCE interfered with G0/G1, S, and G2/M stages of the cell cycle of glioblastoma, and Annexin/PI staining indicated a dose-dependent increase in cell death, confirming the toxic nature of CCE on LN229 cells. These findings suggest that cassava cyanide extract has potential as an antineoplastic agent against glioblastoma cells, which is an aggressive and difficult-to-treat type of brain cancer. However, it is important to note that the study was conducted in vitro, and further research is necessary to assess the safety and efficacy of CCE in vivo. Additionally, it is essential to establish the optimal dose and potential side effects before considering its use as a therapeutic agent. [Display omitted] •Isolation of bioactive compounds from cassava leaves.•Estimation of cassava cyanide extract.•Study on the cell viability on exposure to extract.•Cytoskeletal analysis by Rhodamine Phalloidin staining.•Flow cytometric analysis of Live dead assay.