Highly selective and sensitive determination of doxycycline integrating enrichment with thermosensitive magnetic molecular imprinting nanomaterial and carbon dots based fluorescence probe

• Published in: The Science of the total environment 2023-10, Vol.896, p.165257-165257, Article 165257
• Main Authors: Li, Zhi, Li, Shuangying, Li, Yanhui, Liu, Menghua, Jiang, Liushan, Niu, Jinwen, Zhang, Yue, Zhou, Qingxiang
• Format: Article
• Language: English
• Subjects: Carbon dots; Doxycycline; Fluorescence spectrometry; Thermosensitive magnetic molecularly imprinted polymers
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2023.165257

Doxycycline (DOX), a typical tetracycline antibiotic, is widely used because of its excellent antibacterial activity. To develop effective method for DOX has attracted much more attention. Herein, a new detection technology integrating magnetic solid phase extraction (MSPE) based on thermosensitive magnetic molecularly imprinted polymers (T-MMIPs) and fluorescence spectrometry based on carbon dots (CDs) was established. Thermosensitive magnetic molecularly imprinted polymers (T-MMIPs) was designed for selective enrichment of trace DOX. The synthesized T-MMIPs showed excellent selectivity for DOX. The adsorption performance of T-MMIPs varied with temperature in different solvents, which could achieve the enrichment and rapid desorption of DOX. In addition, the synthesized CDs had stable fluorescent property and better water-solubility, and the fluorescence of CDs was significantly quenched by DOX due to the internal filtration effect (IFE). Under the optimized conditions, the method resulted in good linearity over the range from 0.5 to 30 μg L−1, and the limit of detection was 0.2 μg L−1. The constructed detection technology was validated with real water samples, and excellent spiked recoveries from 92.5 % to 105.2 % were achieved. These data clearly indicated that the proposed technology was rapid, highly selective, environmentally friendly, and possessed significant potential application and development prospects. [Display omitted] •T-MMIPs exhibited good adsorption and desorption ability with temperature change.•The fluorescence quenching of CDs by DOX was contributed to IFE mechanism.•The established MSPE-FL method was reliable for detection of DOX in water.•Proposed method provided low detection limit and high sensitivity