Integrated multifunctional nanoplatform for fluorescence detection and inactivation of Staphylococcus aureus

• Published in: Food chemistry 2023-12, Vol.428, p.136780-136780, Article 136780
• Main Authors: Jiao, Jing-bo, Kang, Qing, Cao, Jiang-li, Zhang, Shuai-qi, Ma, Chen-jing, Lin, Tong, Xiao, Ze-hui, Zhao, Chu-min, Du, Ting, Du, Xin-jun, Wang, Shuo
• Format: Article
• Language: English
• Subjects: Biosensing Techniques; Cascaded signal amplification; Copper; DNA, Single-Stranded; Fluorescence detection; Humans; Inactivation; Integrated multifunctional nanoplatform; Limit of Detection; Nucleic Acid Amplification Techniques; Staphylococcus aureus; Staphylococcus aureus - genetics
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2023.136780

[Display omitted] •One-step cascade signal amplification simplifies detection procedures.•ssDNA-Cu NPs have excellent fluorescence response and sterilization performance.•The platform detection sensitivity is 5.2 CFU mL−1 for Staphylococcus aureus. Foodborne illness caused by Staphylococcus aureus (S. aureus) has posed a significant threat to human health. Herein, an integrated multifunctional nanoplatform was developed for fluorescence detection and inactivation of S. aureus based on cascade signal amplification coupled with single strand DNA-template copper nanoparticles (ssDNA-Cu NPs). Benefiting from reasonable design, one-step cascade signal amplification was achieved through strand displacement amplification combined with rolling circle amplification, followed by in-situ generation of copper nanoparticles. S. aureus detection could be performed through naked eye observation and microplate reader measurement of the red fluorescence signal. The multifunctional nanoplatform had satisfactory specificity and sensitivity, achieving 5.2 CFU mL−1 detection limit and successful detection of 7.3 CFU of S. aureus in spiked egg after