Cucurbita pepo contains characteristic proteins without a signal peptide in the xylem sap

• Published in: Journal of plant physiology 2023-08, Vol.287, p.154038-154038, Article 154038
• Main Authors: Inui, Hideyuki, Minic, Zoran, Hüttmann, Nico, Fujita, Kentaro, Stoykova, Petya, Karadžić, Ivanka
• Format: Article
• Language: English
• Subjects: Cucurbita pepo; Major latex-like protein; Proteomic analysis; Root protein; Secretion; Xylem sap protein
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2023.154038

Xylem sap is a fluid that transfers water and nutrients from the rhizosphere. This sap contains relatively low concentrations of proteins that originate from the extracellular space among the root cells. One of the characteristic proteins in the xylem sap of the Cucurbitaceae family, which includes cucumber and zucchini, is a major latex-like protein (MLP). MLPs are responsible for crop contamination through the transport of hydrophobic pollutants from the roots. However, detailed information on the content of MLPs in the xylem sap is not available. Proteomic analysis of root and xylem sap proteins from the Cucurbita pepo cultivars Patty Green (PG) and Raven (RA) showed that the xylem sap of cv. RA, a high accumulator of hydrophobic pollutants, contained four MLPs that accounted for over 85% of the total xylem sap proteins in this cultivar. The xylem sap of PG, a low accumulator, mainly contained an uncharacterized protein. The amount of each root protein between the PG and RA cultivars was significantly and positively correlated in spite of being with and without a signal peptide (SP). However, the amount of xylem sap proteins without an SP was not correlated. These results suggest that cv. RA is characterized by MLPs in the xylem sap. •Proteomic analysis revealed characteristic proteins in the Cucurbita pepo.•Major latex-like proteins were detected in a high accumulator of pollutants.•An uncharacterized protein was contained in a low accumulator of pollutants.•There were many leaderless secreted proteins in the xylem sap of C. pepo.•Xylem sap proteins characterized a high accumulation of pollutants in C. pepo.