Comparative study of three novel ion exchange resins with DEAE-cellulose for the purification of Trypanosoma evansi

• Published in: Analytical biochemistry 2023-09, Vol.676, p.115226-115226, Article 115226
• Main Authors: Marques, Júlia, das Neves, Gabriella Bassi, Ungri, Amanda Martins, de Souza Franco, Cíntia, Galdino, Nayane Alves de Lima, Ribeiro, Brenda Guedes, Borges, Gabriela Kaiser, Miletti, Luiz Claudio
• Format: Article
• Language: English
• Subjects: Chromatographic matrix; Flow cytometry; Hemoparasite purification; Ion exchange
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/j.ab.2023.115226

Ion exchange chromatography is a method that uses the different surface charges of trypanosomes and blood cells to separate them. This makes it possible to use molecular and immunological methods to diagnose or study these protozoans. DEAE-cellulose resin is commonly used to perform this method. The goal of this study was to compare three novel chromatographic resins designated as PURIFICA™ (Y-C2N®, Y-HONOH®, and Y-CNC3®). The resins were evaluated based on their ability to isolate the parasite, purification time, examination of parasite viability and morphology, and trypanosome recovery potential after passing through the columns. In terms of the evaluated parameters, there was no significant difference between DEAE-cellulose and the three tested resins in most experiments. However, PURIFICA™ (Y-C2N®, Y-HONOH®, and Y-CNC3®) resins are less expensive and easier to prepare than DEAE-Cellulose, making them an alternative for the purification of Trypanosoma evansi. [Display omitted] •Purification by PURIFICA™ resins preserves the vitality of T. evansi.•Morphology of Trypanosoma evansi is maintained.•Trypanosoma evansi purified through resins PURIFICA™ retains its infectious potential.